寡糖的毛细管电泳分析  

Analysis of Oligosaccharides by Capillary Electrophoresis

作　　者：常理文[1] 腰锐锋 郭勍[1] 严峰[1] 

机构地区：[1]中国科学院化学研究所,北京100080

出　　处：《分析化学》1994年第10期975-979,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助课题

摘　　要：多种寡糖经α-萘胺衍生化后,用硼砂作为电泳介质,实现了高效毛细管电泳分离.比较了毛细管区带电泳(CZE)和胶束毛细管电动色谱(MECC)分离寡糖α-萘胺衍生物的电泳行为,对影响分离度的诸因素进行了考察,选择了最佳分离条件.A method for the analysis of oligosaccharides was described using capillary elec-trophoresis. The oligosaccharides were derivatized with naphthylamine, and separated by high-performance capillary electrophoresis in the borax buffer solution as carrier. A comparison between capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chomatography (MECC) of oligosaccharides-ot-naphthylamine was made. The results indicate that the separation of oligosaccharides-a-naphthylamines is better by MECC than by CZE. The optimum conditions for the separation were given.

关 键 词：寡糖 毛细管电泳 MECC 萘胺 

分 类 号：O629.11[理学—有机化学]