对微量锰在兔体内药时曲线的监测研究  

Catalytic Kinetic-Fluorometric Determination of Microamounts of Manganese and the Drug-Time Curve in Rabbit Blood

作　　者：庞志功[1] 汪宝琪[1] 汪丛莹 

机构地区：[1]中国医科大学药学系

出　　处：《分析试验室》1994年第1期20-23,共4页Chinese Journal of Analysis Laboratory

基　　金：国家中医药管理局科研基金

摘　　要：本文以催化动力学分析法为监测手段，利用Ｍｎ￣（２＋）离子对ＫＩＯ＿４－ＲｈＢ反应体系的催化作用，在使用ｐｈｅｎ为活化剂的条件下，顺利实现对兔全血中微量锰的荧光测定，绘制药时曲线，效果良好。A Catalytic k inetic fluorometric methed for the determination of microamounts of manganase has been developed based on the catalytic effect of Mn on the oxidation of rhedamine B by KIO_4 by using o-phenanthroline as the activator.The relative standard deviation is 3.62% at the concentration level of 4×10￣(-9)g/mL with a detection limit of 4.3×10￣(-10)g/mL,The average recovery is 99.76%.This method has been used for the establishment of the drug- time curve in rabbit blood.The mechanism of the catalytic reaction has also been discussed.

关 键 词：催化动力学 锰 药时曲线 兔 荧光 

分 类 号：R965[医药卫生—药理学]