混输脾基质细胞与骨髓细胞悬液在小鼠大剂量辐射损伤修复中的作用  

THE EFFECT OF MIXED INJECTION OF SPLENIC STROMAL CELL SUSPENSION AND BONE MARROW CELLS ON THE RECOVERY OF LARGE DOSE RADIATION INJURY IN MICE

作　　者：于洪臣[1] 凌翎[1] 李勇 张英姬 孟洪 王跃宇[1] 张红梅[1] 刘及[1] 

机构地区：[1]白求恩医科大学预防医学院

出　　处：《辐射研究与辐射工艺学报》1994年第1期40-43,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金

摘　　要：Ｃ５７ＢＬ／６／小鼠经６０Ｃｏγ射线一次整体照射８Ｇｙ后，分成三组：（１）单纯照射８Ｇｙ组。（２）照射后单纯骨髓移植组，（３）照射后混输脾基质细胞（ＳＳＣ）与骨髓细胞（ＢＭＣ）组，以观察骨髓移植和回输脾基质细胞对大剂量照射后受体存活率及造血干细胞（ＣＦＵ－Ｓ）的影响，并初步探讨其可能的机理。结果表明：单纯骨髓移植组受体存活率比单纯照射组高出２７％；而照射后混输ＳＳＣ组之存活率比单照射组高４７％，比单纯骨髓移植组高２２％（Ｐ＜０．０５）。混输ＳＳＣ可明显提高脾ＣＦＵ－Ｓ、ＣＦＵ－Ｆ及ＡＮＡＥ染色（＋）（－）细胞数。在体外混合培养中脾基质细胞对实质细胞增殖起支持与调节作用。The paper reported that C57 BL/6 mice after exposure to 8Gyγ-rays irradiation were divided into three groups: (1) the control group (8 Gy only),(2) the group with bone marrow cell (BMC) injection only, (3) the group withmixed injection of BMC and splenic stromal cell (SSC) which cultured in order toobserve the effect of bone marrow transplantation and injection of SSC on the survival rate of recipients and on the number of hemopoietic stem cells (CFU--S) afterlarge dose irradiation and study its mechanism. The results of experiments suggestedthat the survival rate of recipients of the group with BM cells injection only was27% higher than that of the control group, but that of the group with SSC mixedinjection was 49% higher than that of the control group and 22% higher than thatof the group with BMC injection only. The CFU--S shows that mixed SSC injectionimprove the number of CFU--S, CFU--F and ANAE[ +] [-] lymphocyte in spleen significantly. The cocultivation of spleenocytes and SSC in vitro showed the spleen stromal cells surport and regulate their parenchyma cells.

关 键 词：脾基质细胞 骨髓细胞 放射损伤 

分 类 号：R817.74[医药卫生—影像医学与核医学]