利用微生物转化丙烯腈成丙烯酰胺的研究Ⅱ、产腈水合酶菌种的发酵条件的研究  

STUDY ON CONVERSION OF ACRYLONITRILE TO ACRYLAMIDE BY MICROORGANISMS Ⅱ. FERMENTATION CONDITIONS OF NITRILE HYDRATASE PRODUCING STRAIN

作　　者：孙韦强[1] 张延[1] 余正齐[2] 汪莉[2] 杨钧[2] 尹光琳 李族光 

机构地区：[1]上海交通大学生物科学与技术系 [2]石油化工科学研究院

出　　处：《石油学报（石油加工）》1989年第1期79-84,共6页Acta Petrolei Sinica(Petroleum Processing Section)

摘　　要：对恶臭假单胞杆菌(Pseudomonas putida)JP-1产酶条件进行了研究。结果表明,腈水合酶为诱导酶.该菌株能利用丙腈作唯一的碳、氮源,附加碳源对产酶没有促进作用,但附加玉米浆对产酶则有明显的促进作用.丙腈和丙酰胺是腈水合酶的有效诱导物.Co^(+2)对产酶有明显抑制作用,其它金属离子对产酶无促进作用,JP-1菌产酶最适培养基组成为(%);玉米浆0,15,丙腈0,25(V/V),K_2HPO_4·3H_2O 0.2,NaCl 0.1,MgSO_4·7H_2O 0.02,微量元素0.1(V/V).在pH7.0和28℃条件下培养48h后测得每毫升发酵液酶活为为3.76单位(即16035μg丙烯酰胺/h).The condition for producing nitrile hydratase by strain JP-1 was investegated. The results suggests that nitrile hydratase is an induced enzyme. The strain JP-1 could utilize propionitrile as sole source of carbon and nitrogen. Enzyme formation was not enhanced by additional carbon sources. Added corn steep liquor could remarkably promote enzyme formation. Propionitrile and propionamide were found to be effective inducers for enzyme formation. Enzyme formatoin was considerably inhibited by Co^(++) ion. when the organism grew on a suitable medium consisting of 0.15% corn steep liquor, 0.2% K_2HPO_4·3H_2O, 0.1% NaCl, 0.02% MgSO_4·7H_20, 0.25%(V/V)propionitrile and 0.1%(V/V) trace elements, at pH 7.0 and 28℃ for 48 h, about 3.76 units/ml (or 16035 μg acrylamide/h) of acrylamide was obtained.

关 键 词：微生物 丙烯腈 丙烯酰胺 

分 类 号：TQ225.261[化学工程—有机化工]