蜀柏毒蛾核多角体病毒生物活性测定  

Bioassay of the Nuclear Polyhedrosis Virus of the Lymantriid Parocneria Orienta Chao

作　　者：陈顺立[1] 施和清 李友恭[1] 程保[1] 

机构地区：[1]福建林学院林学系,永安市半村林业采育场

出　　处：《福建林学院学报》1994年第1期58-62,共5页Journal of Fujian College of Forestry

基　　金：福建省林业厅资助

摘　　要：以不同浓度病毒悬液浸渍柏木叶，饲喂感染３龄初蜀柏毒蛾幼虫，感染浓度与死亡机率值回归方程式为ｙ＝３．３３５＋０．３１４ｘ；ＬＣ５０值为２．０３６×１０５ＰＩＢ／ｍｌ．以４×１０６、４×１０７和４×１０８ＰＩＢ／ｍｌ３种浓度病毒感染３龄初幼虫，其ＬＴ５０值分别为７．８４、７．６１和６．８５ｄ．试验表明，同一浓度病毒（４×１０７ＰＩＢ／ｍｌ）对不同龄期幼虫的感染力不同．幼虫死亡率随龄期增大而降低，从２龄的８９．５％降到５龄的６７．０７％；ＬＴ５０值随着龄期增大而延长，从２龄的６．８８ｄ增加到５龄的８．４５ｄ．温度对幼虫感病死亡速度有一定影响，但对总死亡率影响不大．病毒在冰箱中（４℃）保藏一年，对其活性影响不大，而在室温下保藏一年，其感染力下降４６．３％～５４．７％．Early third-instar larvae were fed on Chinese weeping cryprese leaves contaminated with different concentration of polyhedral inclusion bodies (PIB) of PoNPV. Theregressive equation between concentration and mortality was y=3.335+0.314x.The LC50values was 2.036×105PIB/ml.The LT50 values for the virus concentration of 4×106, 4×107and 4×108PIB/ml were 7.84,7.61 and 6. 85 days respectively.Tests of larval suspensionindicated that mortality decreased as the larvae grew from 89.5% of second-instar larvaeto 67.07% of fifth-instar larvae. The LT50 values increased with the increase of instar,from 6.88 days of second-instar larvae to 8. 45 days of fifth-instar larvae. Temperature hadno influence upon the total mortality but it did affect the death rate. PoNPV slfored for oneyear in refrigerator(4℃ ) did not lose its activity,but that stored for one year at room temperature lost 46. 3～54.7% of its activity.

关 键 词：蜀柏毒蛾 多角体病毒 生物活性 

分 类 号：S763.420.6[农业科学—森林保护学]