特定电磁波照射对家兔体外受精的影响  

Effects of Specific Electromagnatic Wave on in Vitro Fertilization of Rabbit

作　　者：黄依明[1] 安民[1] 

机构地区：[1]福建农业大学动物科学系,北京农业大学动物科学技术学院

出　　处：《福建农业大学学报》1994年第2期213-215,共3页Journal of Fujian Agricultural University

摘　　要：家兔精液用特定电磁波（ＴＤＰ）照射３ｍｉｎ，精子体外获能采用ｍ—ＨＩＳ（１５ｍｉｎ）＋ｍ－ＤＭ（２－４ｈ）．家兔卵母细胞经ＴＤＰ照射５ｍｉｎ并体外培养１０－１２ｈ．家兔的卵泡卵母细胞经体外受精和体外培养的结果表明，经ＴＤＰ照射的精液与未经ＴＤＰ照射的卵母细胞的体外受精，原核形成率达６４．７％，对照织为４４．４％，两者差异显著（Ｐ＜０．０５），２－细胞以上的受精卵发育率分别为５６．８％和５０％（Ｐ＞０．０５），经ＴＤＰ照射的卵母细胞与未经ＴＤＰ照射的精液的体外受精，原核形成率达６３．７％，对照组为４６．７％，两者差异显著（Ｐ＜０．０５），２－细胞以上的受精卵发育率分别为５８．９％和４７．２％（Ｐ＞０．０５）、用ＴＤＰ照射精液或卵母细胞能显著提高其体外受精率．Group one: rabbit semen was radiated with Specific Electromagnetic Wave (TDP) for 3 minutes and sperm capacitation was carried out with m-HIS for 15 minutes, and in m-DM for 2-4 hours, then fertilited with normal oocytes in vitro. Group two: normal oocytes were radiated with TDP for 5 minutes and cultivated for 10-12 hours, and then fertilited with normal sperm in vitro. The results showed that the rate of pronucleus formation was 64.7% in treated group and 44.4% in the control (P< 0.05) and the rate of embryo development was 56.8% and 50.0% respectively (P>0.05) in group one. The rate of pronucleus formation was 63.7% in treated group and 46.7% in the control (P<0.05), the rate of embryo development was 58.9%and 47.2% respectively (P>0.05). The results indicated that fertilitation rate in rabbit was significantly incresed by the TDP radiation to semen or oocytes.

关 键 词：兔 特定电磁波 体外受精 家兔 

分 类 号：S829.1[农业科学—畜牧学]