胸腺五肽（TP─5）的固相合成及其对人血液若干生化指标的影响  

THE SYNTHESIS OF THYMOPENTIDE(TP─5)AND ITS SOME INFLUENCESTO BIOCHEMICAL EFFECTS IN HUMAN BLOOD

作　　者：杨国玲[1] 王勤[1] 王珠银 胡晓愚 

机构地区：[1]兰州大学生物系

出　　处：《甘肃科学学报》1994年第1期36-40,共5页Journal of Gansu Sciences

摘　　要：采用多肽固相合成法合成了胸腺生成素Ⅱ（Ａｒｇ─ｌｙｓ─Ａｓｐ─Ｖａｌ─Ｔｙｒ─ＯＨ（ＴＰ─５）），经ＨＰＬＣ检查其纯度达９８％以上，氨基酸组成分析与预期结果相一致，质谱法测得分子量与理论值相符合。自愿受试者肌肉注射ＴＰ─５（每次１ｍｇ／支，间日一次，共十次）后，其血液中超氧化物歧化酶（ＳＯＤ）活性提高了１７５％；对血液中超氧阴离子自由基（Ｏ＿￣（－）＿２）的清除率达２０％，并使红细胞免疫复合物花环率和红细胞Ｃ＿３ｂ受体花环率分别提高了２６．３％和２４．３％。Thymopoietin Ⅱ(32─36)OH,Arg─Lys─Asp─Val─Tyr─ON(TP─5)was synthesizedhy the solid phase peptide synthesis and BOC strategy.The purified peptide was judged to behigher than 98%in purity by using HPLC. The data obtained from amino acid analysis and fastatom bombing mass spectrum were in good agreement with theoretical values. Bioassays of blood samples which were obtained from volunteers after i.m. of TP─5(1mg/d. tertian injection,10 times),show the activity of SOD rose up to 175%,the rate of eliminationof superoxide radical(O￣(-)_2)was around 20%,the rosette rate of RBC─C_36 and that of RBC─ICincreased 24.3/and 26.3% in blood samples separately.

关 键 词：胸腺生成素 血液 超氧物歧化酶 

分 类 号：TQ467[化学工程—制药化工]