羟自由基（OH）、超氧阴离子（O_2~－）与线粒体低水平化学发光相关性的研究  

STUDY ON CORRELATION BETWEEN OH,RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA

作　　者：马玉琴[1] 赵克俭[1] 张仲纶[1] 苏震[1] 马斌[1] 郑雁珍[1] 杨光洁 米志勇 陈昕 

机构地区：[1]中国科学院生物物理研究所

出　　处：《感光科学与光化学》1994年第4期312-317,共6页Photographic Science and Photochemistry

基　　金：国家自然科学基金

摘　　要：以线粒体为模式体系，研究了低水平化学发光的过程和机制。实验发现在一定浓度范围（蛋白０－１００毫克／毫升）内，线粒体悬液的发光是随浓度的升高而增强。最适宜的测量条件为３５℃和ｐＨ＝７．Ａ。注入外源性ＯＨ和ＯＨ的清除剂证实，ＯＨ可起动脂质过氧化反应，且￣１Ｏ＿２是构成发光的体内光子源。超氧化物歧化酶（ＳＯＤ）对线粒体发光有调节作用。itochondria was used a sa model system to study the course and mechanism of low-level chemiluminescence. It is discovered that the LCL of mitochondria suspension riseswith its concentration increasing under the definited range (0-100 mg/mL protein). Thebest satisfactory condition is 35℃ and PH=7.4. By adding of exogenous OH and OHcleaner, it is convicted that OH can initiate lipld peroxidation reactions and ￣1O_2 is one ofthe main photon sources. SOD(in vivo) may adjust the LCL from mitochondria.

关 键 词：线粒体 化学发光 羟自由基 超氧阴离子 

分 类 号：O644.16[理学—物理化学]