BALB／c－3T3细胞的体外微核试验  

IN VITRO MICRONUCLEUS ASSAY IN BALB/C-3T3 CELLS

作　　者：顾祖维 

机构地区：[1]上海市劳动卫生职业病防治研究所

出　　处：《工业卫生与职业病》1994年第4期204-206,共3页Industrial Health and Occupational Diseases

摘　　要：利用已建立的ＢＡＬＢ／ｃ－３Ｔ３哺乳类细胞株，对苯并（ａ）芘、环磷酰胺、２－氨基蒽、２．硝基芴、亚硝化煤尘提取物和纸烟烟雾凝聚物，用微核试验作了遗传毒性的测试。结果表明：ＢＡＬＢ／ｃ－３Ｔ３细胞能活化某些前诱变物和前致癌物。看来ＢＡＬＢ／ｃ－３Ｔ３细胞微核试验，不需加外源性活化系统，作为体外遗传毒性测试系统十分有用。ｐ＜０．０１，以下同。表２环磷酰胺诱发ＢＡＬＢ／ｃ－３Ｔ３细胞微核试验结果表３２－氨基蒽和２－硝基芴诱发ＲＡＬＲ／Ｃ－３Ｔ３细胞微核试验结果处理时间为４８ｈ表４亚硝化煤尘和纸烟烟雾提取物诱发ＢＡＴＢ／Ｃ３Ｔ３细胞微核试验结果煤尘组浓度以原有煤尘量表示。５０μｌ纸烟烟雾凝聚物贮备液加入１０ｍｌ培养液。１，２和４支／ｍｌ相当于每平皿加入１／２０，１／１０和１／５支烟的烟雾，处理时间均为４８ｈ。三、讨论细胞中微核的形成是罕见的细胞遗传学现象。体内微核试验中，微核细胞分布呈负二项或泊松分布［１１］。但是，体外微核试验中微核细胞的分布情况尚无报道。根据我们溶剂对照组的资料，大多数实验中均数大于方差，故在这些体外试验中微核细胞的分布似乎并不服从于负二项或泊松分布。为了确定准确的分布类型还需要更多的实验资料。?The genotoxicity of benzo(a)pyrene,cyclophosphamide,2-aminoanthracene,2-nitrofluorene,nitrosated coal-dust extracts,and cigarette-smoke condensate were tested with the in vitro micronucleus assay using an established BALB/c-3T3 mammalian cell line.The results showed that all chemicals and complex mixtures studied induced micronuclei in cells tested.These results indicate that BALB/c-3T3 cells are capable of activating certain promutagens and procarcinogens.It seems,therefore,that the micronucleus assay in BALB/c-3T3 cells without an exogenous activation system may be useful as an in vitro assay for detecting genotoxicity.

关 键 词：BALB/c-3T3 微核 遗传毒性 细胞 

分 类 号：R135.1[医药卫生—劳动卫生]