火焰原子吸收光谱法测定红细胞膜结合铜、锌、铁  

FLAME ATOMIC ABSOPTION SPECTROMETRY DETERMINATION OF COPPER, ZINC AND FERRUM IN HUMAN ERYTHROCYTE MEMBRANE

作　　者：杨根元[1] 徐德选[1] 杭建明[1] 

机构地区：[1]镇江医学院仪器分析教研室,镇江医学院附属医院肺科

出　　处：《光谱学与光谱分析》1994年第2期91-95,共5页Spectroscopy and Spectral Analysis

摘　　要：本文用火焰原子吸收光谱法测定红细胞结合铜、锌、铁含量。测定健康者３４人，膜结合铜、锌、铁平均值分别为０．２３９＋０．１０８μｇ／ｍｇ膜蛋白，１．８６７±０．５６７μｇ／ｍｇ膜蛋白，１２．５３士４．７９μｇ／ｍｇ膜蛋白；肺癌患者３０人，膜结合铜、锌、铁平均值分别为０．２８２士０．５１５６μｇ／ｍｇ膜蛋白，１．５１４士０．４７６μｇ／ｍｇ膜蛋白，１７．５３士３．７７μｇ／ｍｇ膜蛋白。测定结果表明，肺癌组膜结合锌明显低于对照组（ｐ＜０．０１）；膜结合铁明显高于对照组（ｐ＞０．０５）；膜结合钢与对照组比较，无显著性差异（Ｐ＞０．０５）；膜Ｃｕ／Ｚｎ率明显高于对照组（０．０５＞Ｐ＞０．０１）。该方法的回收率膜铜为９７．２５～１０３．０３％，膜锌为９８．７７～１００．２６％，膜铁为９９．０５～１００．１３％。相对标准偏差膜铜小于３．５７％，膜锌小于３．６９％，膜铁小于３．６０％。The present study determine d the amount of binding copper zinc and ferrum in human erythrocyte membrane by using flame AAS. In thirty-four healthy subjects, the average of binding copper, Zinc and ferrum in erythrocyte membrane are 0.239 ± 0. 108 μg/mg,1. 867 ± 0. 567 and 12. 530± 4. 793 μg/mg membrane protein respectively. In thirty patients with lung cancer disease, the average of binding copper, zinc and ferrum in erythrocyte membrane are 0. 282 ± 0. 151 μg/mg, 1. 514 ± 0. 476 and 17. 533 ± 3. 774 μg/mg membrane proteins respectively. The difference between heaethy subjects and patients of membrane zinc. ferrum and membrane Cu/Zn rate are significant. As to the determination method, the rate of recovery varied the membrane copper from 97. 25% to 103. 03%, the membrane zinc from 98. 77% to 100. 26% and the membrane ferrum from 99. 05 % to 100. 13%. The RSD of Cu, Zn, Fe are less than 3. 57%, 3. 69%, 3. 60%, respectively.

关 键 词：原子吸收 红细胞膜 铜 锌 铁 测定 

分 类 号：Q592.1[生物学—生物化学]