电场作用下紫膜中细菌视紫红质的圆二色性研究  

STUDIES ON CIRCULAR DICHROISM OF BACTERIORHODOPSIN IN PURPLE MEMBRANE UNDER THE ACTION OF ELECTRIC FIELD

作　　者：王敖金[1] 胡坤生[1] 孙永泰[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《光谱学与光谱分析》1994年第3期41-44,58,共5页Spectroscopy and Spectral Analysis

基　　金：国家基金委重大项目

摘　　要：垂直于测量光束的外电场对紫膜水悬浮液中的细菌视紫红质的圆二色性有很大影响。５０Ｈ２正弦交流电场作用下产生５６８ｎｍ的正的差圆二色性峰，反向电脉冲串作用下产生５５６ｎｍ左右的负的差圆二色性峰；两峰峰位均未显示有随电压的变化而移动；但是，强度随外加电压的增加而增强；撤去电场后该两峰均即刻消失。分析认为，电场下细菌视紫红质中存在柔性结构变化，这种结构变化也可用于存储信息。The changes occuried in the circular dichroic spectra of bacteriorhodopsin in thepurple membrane aqueous suspensions if an electric field was applied. There was a positive568nm peak of differential circular dichroic spectra under the action of 50Hz AC electricfield, but a negative one at about 556nm due to alternate pulse series. However they disappeared as soon as the field was turned off. It is suggested that the changes of flexible structure occured in the bacteriorhodopsin under the action of electric field, which may be used tostore information.

关 键 词：电场 紫膜 细菌视紫红质 圆二色性 

分 类 号：O629.73[理学—有机化学]