脐带血及胎盘组织人巨细胞病毒的检测  

DETECTION OF HUMAN CYTOMEGALOVIRUS IN CORD BLOOD AND PLACENTAL TISSUE

作　　者：黄树林[1] 李国明[1] 张湘宁[1] 罗晨美[1] 陈坤元 刘仿[1] 

机构地区：[1]广东医学院微生物学教研室,湖北省人民医院

出　　处：《广东医学院学报》1994年第1期15-16,14,共3页Journal of Guangdong Medical College

摘　　要：以核酸杂交法检测了６０份脐血标本及４６份胎盘组织标本中人巨细胞病毒ＤＮＡ同源序列，发现脐血阳性１４份（阳性率为３０．４％），胎盘组织阳性１份（阳性率为２．１７％）。用免疫酶染色法测定６０份脐血的ＨＣＭＶ抗体（６０份标本中４６份与脐血和胎盘组织ＤＮＡ配对），ＩｇＧ阳性４６份（阳性率７ｌ．７％）、ＩｇＭ阳性者ｌ份（阳性率２．２％），两型抗体均为阳性者ｌ份。本结果证实了ＨＣＭＶ能通过胎盘这种传播途径引起宫内感染的已有结论。Homologuous human cytomegalovirus(HCMV) DNA sequence was detected in 60 cases of cord blood and 46 cases of plancenta with nucleic acid dot blot hvbridization. 14 samples of cord blood were positive,the positive rate was 2.17%. One sample of placenta was positive,the positive rate was 2.17%. Anti-HCMV antibodies were detected in 60 cord blood samples, 46 among which were from the same donors of the placentae with immuno-enzymatic staining. 46 were positive for IgG, and one was positive for IgM, one was positive for IgG+IgM. The positive rates for IgG ane IgM antibodies were 7l.73% and 2.2% respectively. HCMV exists ubiquito usely in human population. The results confirmed that HCMV could ultilize placenta as a route of transmission to cause intrauterine infection.

关 键 词：巨细胞病毒 脐血 胎盘 DNA 杂交 

分 类 号：R373.9[医药卫生—病原生物学]