肿瘤细胞三种药敏测试方法的比较研究  

COMPARATIVE STUDY OF THREE KINDS OF CHEMOSENSITIVITY TESTS FOR TUMOR CELLS

作　　者：梁谋[1] 吴波[1] 赵明伦[1] 

机构地区：[1]广东医学院病理生理学教研室,广东医学院生物化学教研室,深圳三九企业集团医药研究所

出　　处：《广东医学院学报》1994年第4期291-294,共4页Journal of Guangdong Medical College

摘　　要：以人早幼粒白血病细胞系ＨＬ－６０为对象，进行了ＭＴＴ法细胞浓度曲线和五种抗癌药物的敏感试验，并与台盼蓝拒染法、￣３Ｈ－ＴｄＲ掺入法进行了比较，结果表明在一定活细胞范围内（１．２５×１０￣４～２×１０￣５），ＭＴＴ法所测光密度与细胞数成线性关系（ｒ＝０．９９８０，Ｐ＜０．０５）；三种方法测定的５－Ｆｕ、ＭＴＸ、ＶＣＲ、Ｈ、Ａｒａ－Ｃ药物敏感性结果一致，ＭＴＴ法灵敏度与台盼蓝拒染法相似（Ｐ＞０．０５），但低于￣３Ｈ－ＴｄＲ掺入法（Ｐ＜０．０５）。The MTT assay was used to run the cell concetration curve test and assess the sensitivity of human promyelocytic leukemia HL-60 cells to five chemotherapeutic agents, and compared with the trypan blue exclusion and 3H-TdR incorporation assay The results showed that there was a linear relationship between the viable cell number and the optical density of the MTT formazan product, when the viable cell number was in the range of 1.25×104～2×105.The result of chemosensitivity testing of three kinds of assays was consistent with 5-Fu、MTX、VCR、H、Ara-C. The sensitivity of the MTT assay was similar to that of the trypan blue exclusion assay (P>0.05)but lower than that of the 3H-TdR incoporation assay(P<0.05)

关 键 词：肿瘤 抗癌药 细胞株 比色法 药物敏感性 

分 类 号：R979.1[医药卫生—药品] R965.3[医药卫生—药学]