毛花猕猴桃愈伤组织诱导与植株再生  

Callus Induction and Plant Regeneration from Actinidia eriantha Benth

作　　者：张远记[1] 钱迎倩[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《广西科学》1994年第4期1-5,共5页Guangxi Sciences

摘　　要：从毛花猕猴桃（ＡｃｔｉｎｉｄｉａｅｒｉａｎｔｈａＢｅｎｔｈ）田间生长的茎段、种子无菌条件下萌发的下胚轴及试管苗的茎段和叶片得到愈伤组织。田间来源的茎段诱导愈伤组织较难，愈伤组织生长缓慢；下胚轴及试管苗茎段和叶片容易产生愈伤组织，愈伤组织生长旺盛。消毒时间、接种方法、基因型和培养基都可能影响到田间来源的茎段愈伤组织产生。在ＭＳ附加玉米素或６－苄氨基嘌呤（ＢＡＰ）的培养基上下胚轴来源的愈伤组织不经转代即分化芽和根。试管苗茎段和叶片在附加玉米素或Ｎ－（２－氯基－４－吡啶基）－Ｎ′－苯基脲（ＣＰＰＵ）的ＭＳ培养基上培养一代也分化出芽、试管苗茎段在附加０．００２５ｍｇ／ＬＣＰＰＵ和０．１ｍｇ／Ｌ吲跺乙酸（ＩＡＡ）的ＭＳ培养基上产生愈伤组织、芽的分化和苗生长都较理想；试管苗叶片则以附加０．０２５ｍｇ／ＬＣＰＰＵ和０．１ｍｇ／ＬＩＡＡ或０．５ｍｇ／Ｌ玉米素和０．１ｍｇ／ＬＩＡＡ的ＭＳ培养基较好。当苗伸长至１ｃｍ或以上时切下，转入ＭＳ基本培养基（大量元素减半）上后可形成完整植株。Calli were induced from stem segments of field-grown plants, elongated hypocotyls, stem segments and leaves of in vitro seedlings of A. eriantha. Callus induction from hypocotyls and explants of in vitro seedlings was much easier and calli grew vigorously, and that from field-grown stem segments was difficult and calli grew slowly. Factors affecting callus induction from field-grown stem segments were found to be disinfection duration, genotypes, and growth regulators used. Adventitious buds and roots differentiated from hypocotyl-derived calli on MS medium supplemented with zeatin or benzyl-aminopurine (BAP) without subculture. Bud and occasionally root differentiation also occured from in vitro seedlings on MS medium supplemented with zeatin or N- (2-chloro-4-pyridyl)-N'-phenylurea (CPPU) with one passage culture. The optimal media for the callus induction, bud differentiation andshoot growth to the stem segments of in vitro seedlings were MS medium supplemented with 0. 0025mg/L. CPPU and 0. 1 mg/L indole-3-acetic acid (IAA) and those to the leaves of in vitro seedlingswere MS medium supplemented with 0. 025 mg/L. CPPU and 0. 1 mg/L IAA or 0. 5 mg/L zeatin and 0. 1 mg/L. IAA. Whole plants regenerated from shoots which were 1 cm in length or longer after transferring onto MS medium with half strength of macro-elements.

关 键 词：毛花猕猴桃 愈伤组织 植株再生 猕猴桃 

分 类 号：S663.9[农业科学—果树学]