软紫草愈伤组织的初步培养  

PRELIMINARY STUDY ON CALLUS CULTURE OF ARNEBIA EUCHROMA

作　　者：王黎[1] 张治国[1] 蔡志光 韩献忠[1] 刘骅[1] 

机构地区：[1]浙江省医学科学院

出　　处：《广西植物》1994年第4期345-348,共4页Guihaia

摘　　要：外植体来源不同的软紫草愈伤组织产生紫草色素的能力各异。６０Ｃｏ－ｒ射线辐照处理后的Ｈ２无性系愈伤组织生长量和色素产生均属上乘。改良ＭＳ基本培养基添加１毫克／升ＫＴ，０．５毫克／升ＩＡＡ，５％（Ｗ／Ｖ）蔗糖对愈伤组织培养较为适宜。马铃薯提取液对愈伤组织生长有明显的促进作用。Callus of various explants of Arnebia euchroma had different ability to produce shikonin. H2 clone which was treated by 60Co-r rays had higher production in both callus yield and shikonin than the others. A modified MS medium with 1 mg/l KT, 0.5 mg/l IAA, 5% sucrose stimulated production of callus and shikonin. Potato extract was benefit to callus production.

关 键 词：软紫草 愈伤组织 紫草素 组织培养 

分 类 号：Q949.777.4[生物学—植物学]