海刀豆、红树DNA的提取  

Extraction of DNA form Canavalima maritima Rhizophora apiculata

作　　者：邓用川[1] 曾驰[1] 徐立新[1] 林栖凤[1] 李冠一[1] 

机构地区：[1]海南大学生物中心生化研究室

出　　处：《海南大学学报（自然科学版）》1994年第1期33-35,共3页Natural Science Journal of Hainan University

基　　金：国家科委;国家海洋局资助

摘　　要：本文介绍了两种简便、快速的ＤＮＡ提取方法，它们不需要特殊试剂、液氮和柱层析系统、超速离心机等贵重仪器。我们以海刀豆和红树两种盐生植物为实验材料，所制得的ＤＮＡ样品经紫外分光光度计检测：其最大吸收波长均为２５８ｎｍ；Ａ＿（２５８）／Ａ＿（２８０）均为１．８２；与Ｓｅｐｈａｒｏｓｅ４Ｂ柱层析分离结果相近；用琼脂糖凝胶电泳检测为均一的谱带。Two simple, quick methods of DNA extraction are introduced here. Special reagentand valuable instruments, such as liquid nitrogen, column chromatography and ultracentrifugeetc. were not required in the extraction. Two kinds of salt-enduring plants-Canavalimamaritima and Rhizophora apiculata were chosen as experimental materials. DNA samplesobtained were checked by ultraviolet spectrophotometer and agrose electrophoresis. Both of theirmaximum absorption wavelength were the same of 258 nm; Both Of their A_(258)/A_(280) ratio were 1.82; the isolation results closed to that by Sepharose 4B. The electrophoresis gel tests showed thatthere was an equal and even one band.

关 键 词：DNA 提取 红树 海刀豆 

分 类 号：Q523.03[生物学—生物化学]