^（211）At标记抗胃癌单抗3H11及其Fab片段在荷人胃癌裸小鼠体内的分布  

TISSUE BEODISTRIBUTION IN NUDE MICE OF THE α-EMITTER ￣(211)At LABELING A MONOCLONAL ANTIBODY 3H11 AND ITS Fab FRAGMENT AGAINST GASTRIC CANCER

作　　者：张叔渊[1] 金建南[1] 刘宁[1] 王娟[1] 罗林[1] 王方元[1] 周懋伦[1] 罗德元[2] 万文徽 董志伟 

机构地区：[1]四川大学原子核科学技术研究所 [2]华西医科大学肿瘤研究所 [3]北京肿瘤防治研究所

出　　处：《核化学与放射化学》1994年第3期166-172,共7页Journal of Nuclear and Radiochemistry

基　　金：国家教委博士点基金资助项目

摘　　要：利用ＨＰＬＣ和ＥＬＩＳＡ法对￣（２１１）Ａｔ－３Ｈ１１的分析鉴定表明，间接亲核标记法得到的产品具有３Ｈ１１的天然性和纯度，并保持其免疫活性。￣（２１１）Ａｔ在荷瘤裸小鼠体内分布的结果表明，￣（２１１）Ａｔ－３Ｈ１１Ｆａｂ和￣（２１１）Ａｔ－３Ｈ１１与胃癌细胞有明显的特异亲合力，注射药物３ｈ后，其胃癌摄取率（％／ｇ）分别为９．４８±０．６５和４．９１±２．０５，前者的Ｔ／ＮＴ值在１２－１４ｈ左右明显大于后者。The quality and immunoreactivity of ￣(211)At labelled 3H11 are measured by HPLC and ELISAassay, respectively. The biodistribution is performed in nude mice bearing subcutaneous hetero-graft of human gastric cancer cells followling,i.v, injection of ￣(211)At-3H11,￣(211)At-3H11Fab andNa￣(211)At￣(211)At,respectively. It is demonstrated that the pharmacokinetics of the astatinated Fab frag-ment appear to be well suited to 7.2h half-life of ￣(211)At,since tumor uptake remains high(～10%/g)from 3 to 14h after i.v,injection.

关 键 词：胃肿瘤 砹211 单克隆抗体 FAB片段 

分 类 号：R979.1[医药卫生—药品]