已知Lr基因小麦在叶锈菌侵染过程中PO活性及其同工酶的变化  

Changes of Peroxidase Activity and Isozymes in the Course of Leaf Rust Infection of Wheat Lines with known Lr

作　　者：王冬梅[1] 王智 杨秀屏 刘娟[1] 韩公社[1] 

机构地区：[1]河北农业大学农学系

出　　处：《河北农业大学学报》1994年第1期1-6,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金

摘　　要：利用２个近等基因系ＴｃＬｒ３和ＴｃＬｒ２６及３个小麦品种洛夫林１０＜Ｌｔ２６＞、阿芙乐尔＜Ｌｒ３＋Ｌｒ２６＞、和洛夫林１３＜Ｌｒ３＋Ｌｒ２６＞分别与２个毒力不同的叶锈菌（Ｐｕｃｃｉｎｉａｒｅｃｏｎｄｉｔａｆ．ｓｐ．ｔｒｉｔｉｃｉ）小种１０—２和冀７７—１，组合成亲和程度不同的品种—小种组合。对侵柒过程中过氧化物酶（ＰＯ）活性及其同工酶谱的变化进行了研究。结果表明，ＴｃＬｒ３与小种互作过程中ＰＯ活性变化是单峰曲线特征，峰值出现在接种后３６ｈ，同时在ＰＯ同工酶谱的变化中表现出ｐ１６．３，６．８处的酶带活性增强，而ＴｃＬｒ２６和其他含Ｌｒ２６的各品种与小种互作过程中ＰＯ活性变化均呈双峰曲线特征，峰Ⅰ，Ⅱ分别出现在接种后３６ｈ和８４ｈ，同时在ｐｏ同工酶谱变化中也有特征性表现。Five wheat lines of cultivars containing different resistant genes (TcLr3, TcLr26, Lorin10[Lr26], Aurora [Lr3+Lr26], and Lovrin13[Lr3+Lr26]) were inoculated with tow leaf rust (Puccinia recondita f. sp. tritici) races 10-2 and ji77-1 resectively to form the combination with different compatibilities. The changes of peroxidase(PO) activity and isozymes in the course of leaf rust infection of wheat lines were studied. The change of PO activity in wheat lines bearing Lr3 showed a single peak (36h. after inoculation). The isozyme bands of PO at pI value of 6. 3 and 6. 8 were induced to increase activity at this wheat line. As for the wheat line and other varieties bearing Lr26, PO activity showed two peaks, which appeared at 36h and 84h after inoculation respectively. When Lr3 and Lr26 were contained in the wheat cv. (Aurora or Lovrin13 ), the Lr26 showed epistasis effect on the Lr3. In the early stages of infection (12-24h after inoculation), the PO activity in the incompatible combination was higher than that in the compatible combination. It can be concluded that the physiological unknown resistant genes in wheat cv.

关 键 词：小麦 近等基因系 叶锈菌 同功酶 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]