小麦愈伤组织原生质体培养高频率细胞分裂及愈伤组织形成  

High Frequency cell Division and callus Formation in the Culture of Protoplast Derived from Embryogenic Callus of Wheat

作　　者：程增书[1] 高志强[1] 方仁[1] 

机构地区：[1]河北省农林科学院粮油作物研究所

出　　处：《华北农学报》1994年第1期1-6,共6页Acta Agriculturae Boreali-Sinica

摘　　要：从小麦ＣＫ８９、１０２、１１３的花药和花冬的约穗诱导愈伤组织的继代培养中，选择致密颗粒型愈伤组织，转入附加２，４－Ｄ２～４ｍｇ／Ｌ改良ＭＳ固体培养基上进行继代培养，６～１０周后形成生长迅速的胚性愈伤组织，这种愈伤组织分离原生质体得率为１～３．７×１０７个／ｇ。原生质体于ＰＣＭ２培养基上琼脂糖包埋培养，３～５天出现第一次细胞分裂，７～１４天进行第二、第三次细胞分裂，两周后形成大量细胞团，细胞分裂频率达２０．３％～７３．３％，４～６周后形成肉眼可见愈伤组织。供试４个基因型中，ＣＫ８９、１０２和花冬均形成再生愈伤组织，仅１１３形成多细胞团。愈伤组织增殖分化实验尚在进行中。The compact and granular calli were selected from anther-induced calli of wheat varieties CK89,102,113 and immature inflorescence-induced calli of Huadong,respectively.While these calli were cultured in the modified MS medium with 2-4mg / L 2,4-D, a friable fast-growing type of embryogenic calli was obtained after 6 to 10 weeks. The high yield protoplasts were isolated from the culture and the protolast yield was 1 to 3.7×107 / g calli.The isolated protoplasts were cultuerd in PCM2 medium solidified with 0.4% agarose.The first division occured 3-5 days after plating. The second and third divisions were identifiable during 7 to 14 days.A large number of cell aggregates emerged after two weeks culture, and the division frequency was 20.3%-73.3%.After 4-6 weeks plating,the microcalli formed.The calli were obtained from wheat varieties CK89, 102 and Huadong, and cell aggregates were obtained from 113.An experiment on calli differentiation is under way.A few factors affecting the protoplast cultures were also studied.

关 键 词：小麦 愈伤组织 原生质体 细胞分裂 

分 类 号：S512.103.5[农业科学—作物学]