应用同工酶进行杏属植物演化关系和分类的研究  

A Study on the Evolution Relationship and Classification of Apricots via Peroxidase Isozyme Zymograms Analysis

作　　者：吕英民[1] 吕增仁[1] 高锁柱[1] 

机构地区：[1]河北农业大学园艺系,张家口农业高等专科学校

出　　处：《华北农学报》1994年第4期69-74,共6页Acta Agriculturae Boreali-Sinica

摘　　要：采用聚丙烯酰胺凝胶电泳技术，对杏属（Ａｒｍｅｎｉａｃａｓｐｐ．）植物５个种和１个类型（仁用杏）的１０２个品种的一年生枝皮过氧化物酶（ＰＯＤ）同工酶进行了测定分析。结果表明，杏一年生枝皮的ＰＯＤ同工酶多态、稳定、活性强，适合于杏的分类；谱型分析支持了这种观点，即杏属植物的进化方式是自然的地理隔离，其演化关系是西伯利亚杏、东北杏、藏杏和梅等种均由最原始的种普通杏（Ａ．ｖｕｌｇａｒｉｓ）进化而来；ＰＯＤ同工酶是鉴定杏属内种间杂种的最理想的遗传标记。试验结果显示，仁用杏是普通杏和西伯利亚杏之间的自然种间杂种。The peroxidase isozymes in one-year-old shoot-bark of Apricots(Armeniaca spp.)were determined via polyacrylamide gel electrophoresis.The experiment results are shown as follows:1.Isozyme band patterns of peroxidase are clear, polymorphic and stable in one-year-old shoot-bark extracts of apricots.It is suitable isozyme as gene marker in apricots.2.Isozyme analysis stands by the points:the evolution mode of Armeniaca spp.is natural geography isolation;A.vulgaris Lam. is the primitive species in Armeniaca spp.;other relative A.mume sieb. have been evolued from A.vulgaris Lam.3.Isozyme of POD is the best gene marker to identify interspecies hybrids. Renyongxing(a group of cultivars for sweet kernel products)is the apricot origin from interspecies hybrid between A.vulgaris Lain.and A.sibirica(L.)Lam.

关 键 词：杏 杏属 同功酶 分类 聚丙烯酰胺 凝胶电泳 

分 类 号：S662.201[农业科学—果树学]