不同生长期上海四膜虫（Tetrahymena　shanghaiensis　S1）的同工酶的比较研究  

^Comparative Investigation on Isoenzymes in Different Growth of Tetrahymena shanghaiensis S1

作　　者：侯连生[1,2] 许新[1,2] 彭水根 沈锡祺[1,2] 

机构地区：[1]华东师范大学生物学系 [2]景德镇教育学院

出　　处：《华东师范大学学报（自然科学版）》1994年第4期85-89,共5页Journal of East China Normal University(Natural Science)

基　　金：校基金

摘　　要：用高分辨力的聚丙烯酰胺凝胶电泳分析和比较了不同生长期的上海四膜虫的五种同工酶。发现对数期细胞和衰老期细胞的酸性磷酸酶同工酶，酯酶同工酶和苹果酸脱氢酶同工酶及其它的同工酶的蛋白条带有明显差异。对数生长期细胞的同工酶活力和数量明显高于衰老期细胞的同工酶的活力和数量。说明同工酶不但是细胞代谢的调节者，亦能作为细胞衰老的一种指标。The isoenzymes of acid phosphatase, alkaline phosphatase, malate dehydrogenase,glutamate dehydrogenase and esterase of axenic culture of Tetrahymena shanghaiensis S1 in log phase and aging phase are studied by means of polyacrylamide electrophoresis. The distinct difference exists in each enzymic pattern of polyacrylamide gel electrophoresis. The activity and quanties of each isoenzyme under log phase of growth are obviously higher than that of each isoenzyme under aging phase of growth. It seems that changes of isoenzyme pattern may be used as an index of cell aging.

关 键 词：上海四膜虫 凝胶电泳 对数生长期 衰老期 同工酶 

分 类 号：Q959.117[生物学—动物学]