蒽醌及黄酮类化合物与牛血清的蛋白结合反应的光谱研究  

Spectroscopic Studies on Binding of Anthraquinones and Flavonoids to Bovine Serum Albumin

作　　者：张保林[1] 王文清[1] 袁荣尧[1] 杨俊林[2] 白凤莲[2] 

机构地区：[1]北京大学技术物理系 [2]中国科学院化学研究所,北京100080

出　　处：《化学学报》1994年第12期1213-1217,共5页Acta Chimica Sinica

摘　　要：用停留技术研究了大黄素与牛血清白蛋白(BSA)结合反应的动力学,发现在25℃,pH10.0时结合在100ms即可达到平衡.大黄素等7种蒽醌及黄酮类化合物与BSA结合后,可见区最大吸收波长红移,吸收强度增加.荧光光谱研究表明,这些化合物对BSA荧光有很强的猝灭作用.根据猝灭结果,求出了它们与BSA的结合常数,并基于F(?)rster非辐射能量转移机理,计算了它们的第一结合部位与BSA中212-色氨酸残基的距离.提出了蒽醌及黄酮类化合物与BSA形成复合物的结构模型.Stopped flow studies show that the binding equilibrium of emodin to bovine serum albumin (BSA) is established within 100ms. The absorption maxium of these compounds exhibit red shift upon binding and the absorbance enhanced. The fluorescence quenching associated with the binding of these compounds to BSA was used to measure binding constants, and the distance of Trp-212 to the first binding site were calculated, on the basis of the energy transfer between BSA and bound complexes. An allosteric domain model of emodin-BSA complex was postulated.

关 键 词：蒽醌 黄酮 牛血清白蛋白 结合 

分 类 号：O625.463[理学—有机化学]