线扫极谱法快速测定鱼组织中的金属硫蛋白  

RAPID DETECTION OF METALLOTHIONEIN IN FISH TISSUES BY LINEAR SWEEP POLAROGRAPHY

作　　者：铁锋 潘爱华[1] 李令媛[1] 茹炳根[1] 王英彦[2] 

机构地区：[1]北京大学生物系,北京100871 [2]北京市环境保护科学研究所,北京100037

出　　处：《环境化学》1994年第1期80-86,共7页Environmental Chemistry

基　　金：获863国家高科技;北京市自然科学基金资助

摘　　要：本文报告了用线扫极谱法测定鱼组织中金属硫蛋白(MT)的分析方法,其主要步骤是将鱼组织制成匀浆,加热变性除去杂蛋白,离心后取上清液直接加入钴氨液中,记录-1.46V(νS SCE)处峰形,由峰高求得MT浓度.本文给出MT标准波形,凝胶过滤分离-峰电流图谱,干扰因素及其浓度,检出量和回收率,最后求得鲫鱼六种组织中MT的本底值和经Cd(Ⅱ),Hg(Ⅱ)和Cr(Ⅵ)诱导后的实测值.结果表明本方法具有快速、灵敏和简易等特点.鱼组织(尤其是肝)中MT含量变化反映水体中重金属污染状况,因此可能用于环境监测.The detection technique of metallothionein (MT) in fish tissues by -using linear sweep polarograph(LSP) has been established. The main procedure is as follow; the tissues were homogenized, heated and centrifuged; then the supernatant was directly added in ammoniacal solution of Co( Ⅱ ) salt, followed by scanning from - 0.05 to - 1.80V. The content of MT was obtained according to the peak height at - 1.46V(vs SCE), In this work, MT standard polarogram, Sephacryl S-100 gel filtration chromatography peak current curve, interferent substance and their concentration, detection limit and recovery(%) were reported. In the end, the MT contents of six tissues from fish before and after the treatment with Cd( Ⅱ ), Hg ( Ⅱ ) and Cr( Ⅵ) were investigated. The results show the technique of LSP has the advantage of rapid, sensitive and convenient. The method is adequate for environmental monitoring.

关 键 词：金属硫蛋白 线扫极谱法 鱼类 污染 

分 类 号：X503.225[环境科学与工程—环境工程]