蛇毒抗栓酶对氧自由基致血小板变化的影响  

Effect of Snake Venom Antithrombus Enzyme on The Change of Platelet Induced by Oxyradicals

作　　者：陈丽英[1] 许澍淮[1] 金淑敏[1] 张抒扬[1] 金兰[1] 

机构地区：[1]中国医学科学院基础医学研究所病理生理研究室,协和医院内科心组

出　　处：《基础医学与临床》1994年第2期34-37,共4页Basic and Clinical Medicine

摘　　要：次黄嘌呤／黄嘌呤氧化酶体系产生的氧自由基在体外能明显增加血小板膜微粘度、脂质过氧化代谢产物丙二醛含量、血小板胞内游离钙浓度和血小板５－羟色胺的释放量。０．０３ｕ／ｍｌ的蛇毒抗栓酶明显抑制前二种效应，但促进血小板释放５－羟色胺，增加血小板胞内游离钙浓度。结果表明蛇毒抗栓酶对血小板膜有保护作用。he experiments were performed with rabbit platelet suspension(PSS)to studythe effect of snake venom antithrombus enzyme(SVATE)on platelets in vitro.In thispaper,the production of free radicals derived from hypoxanthine(HX) /xanthineoxidase(XO) ,platelet malondialdehyde (MDA) ,platelet membrane microvisco-sity,5-HT released from platelet and platelet[Ca2+]i were respectively measur-ed.The experiments were divided into 4 groups:1.PSS(control);2.PSS + HX/XO; 3.PSS + SVATE;4.PSS + SVATE + HX/XO.The results showed that pla-telet membrane microviscosity,the production of MDA and 5-HT and platelet[Ca2+]i were significantly increased by oxyradicals.The intervention ofSVATE can significantly inhibit the two former effecls of oxyradicals and incre-ase 5-HT release of platelet and platelet[Ca2+]i.The results indicate thatSVATE has a protective effect on platelet membrane.

关 键 词：氧 自由基 血小板 蛇毒抗栓酶 

分 类 号：R973.2[医药卫生—药品]