乙肝病毒前S_2抗原发光酶免疫方法的临床应用  

Study of Clinical Application of Luminescence Enzyme Immunoassay for Pre-S2 Antigen

作　　者：陈紫榕[1] 陈菁[1] 刘小朋[1] 李龙洋[1] 林华[1] 周善建 张国安[1] 

机构地区：[1]空军福州医院,福建医学院

出　　处：《基础医学与临床》1994年第2期55-58,共4页Basic and Clinical Medicine

摘　　要：建立乙肝病毒前Ｓ２抗原发光酶免疫方法，检测ＨＢＶ感染者血清、肝组织及其细胞器中的前Ｓ２，结果与ＨＢｅＡｇ、ＨＢｃＡｇ、ＨＢＶＤＮＡ等复制指标一致，某些肝内ＨＢＶＤＮＡ阴性者ＰＳ２阳性。这提示前Ｓ２是ＨＢＶ活跃复制的指标。前Ｓ２在肝内主要存于微粒体和核糖体中。前Ｓ２抗体阳性，与肝细胞破坏有关，急性和慢迁肝预示恢复，慢活肝、肝硬化和肝癌预示恶化。本法集发光反应的快速敏感及免疫反应的特异性于一身，敏感性比ＥＬＩＳＡ强５倍，重复性好，无放射性和致癌性。t is the purpose o this article to clinical application and use of luminescenceenzyme immunoassay( LEIA) for Pre-S2 antigen. The method combined rapidity,sensitivity of Luminescence with specificity of immunoassay, radioactiveless and careinogenieityless. The positve rate of Pre-S2 antigen by LEIA (54.4%)washigher than by ELISA(52.1%).The Pre- S2 in the Serum, Liver and its orga-nelle of patient with HBV infection was determined by LEIA,The results showedthat it was strongly related to HBeAg,HBcAg,HBV DNA,anti- HBcIgM. Althoughmost patients were strongly positive for Pre-S2 antigen,some patients with positivePre-S2 antigen did not have detectable HBV DNA and vice versa,Our data showthat assay of Pre-S2 antigen in the serum liver and its organelle by LEIA is moreproficient than by ELISA and that it annot be replaced strological assays of HBeAgand HBV DNA

关 键 词：乙型肝炎病毒 前S2抗原 酶免疫试验 

分 类 号：R373.2[医药卫生—病原生物学]