人参二醇组皂甙对大鼠心肌细胞钙通道阻滞作用  

The Calcium Channel Blockade Action of Panaxadiol Saponins in Rat Cardiomyocytes

作　　者：江岩[1] 王小明[1] 王中峰[1] 钟国赣[1] 张文杰[1] 

机构地区：[1]白求恩医科大学生理中心实验室

出　　处：《基础医学与临床》1994年第2期47-50,共4页Basic and Clinical Medicine

摘　　要：分离Ｗｉｓｔａｒ大鼠乳鼠的心窒肌细胞．向培养基中分别加入人参二醇组皂甙１５００ｕｇ／ｍｌ，钙通道阻滞剂异博定３７．５ｕｇ／ｍｌ或钙通道激动剂ＢＡＹＫ８６４４５μｍｍｏｌ／Ｌ。用细胞封接式膜片钳技术，记录加药前后Ｌ、Ｔ、Ｂ三型钙通道的单通道活动。确证了人参二醇组皂甙的钙通道阻滞作用．并揭示其作用机制，在于使钙通道的开放时间缩短与开放概率减少．istar rat ventricular myocardiocytes were isolated.Panaxadiol saponins(PDS)1500ug/ml,calcium channel blocker verapamil 37.5ug/ml or calcium cha- nnel activator BAY K 8644 5μmmol/L were added into the bath solution separa- tely.The single channel activities of L,T and B type calcium channels were re- corded before and after administration, by using the patch clamp technique in cell- attached configuration.The calcium channel blockade effect of PDS was exactly demonstrated,in which the decrease in both the open time and the open-state pro- bability of the calcium channels was observed.

关 键 词：人参 二醇组皂甙 钙通道阻滞剂 

分 类 号：R972[医药卫生—药品]