干扰素－α对兔离体的卵巢颗粒细胞孕酮生成的影响  

Effect of Interferon-a on Progesterone Production in Rabbit Ovarian Granulosa Cells

作　　者：宿滨[1] 王启发[1] 姜良[1] 马青年[1] 姚兰春[1] 

机构地区：[1]哈尔滨医科大学实验动物学部

出　　处：《基础医学与临床》1994年第3期30-32,共3页Basic and Clinical Medicine

摘　　要：本研究应用离体培养的兔卵巢颗粒细胞观察了ＩＦＮ－ａ对孕酮生成的影响。细胞预培养２４ｈ后，用ＩＦＮ－ａ（５０～８００Ｕ／ｍｌ）处理培养的颗粒细胞１２ｂ刺激孕酮的生成并呈剂量依赖关系。在ＩＦＮ－ａ加入培养细胞后３、１２和２４ｈ．刺激作用非常明显，但刺激作用的幅度在３和１２ｈ较大，分别为对照组的２４和２０倍。此外，ＩＦＮ－ａ刺激离体兔颗粒细胞孕酮生成的作用被同时加钙螫合剂ＥＧＴＡ（１ｍｍｏｌ／Ｌ）或钙通道阻断剂异搏定（ｖｅｒ）２０和２００μｍｏｌ／Ｌ所抑制。本研究结果表明，ＩＦＮ－ａ刺激离体兔卵巢颗粒细胞孕酮的生成，其作用依赖于细胞外的Ｃａ２＋浓度。he present study was designed to examine the possible role of IFN-a in theregulation Of rabbit ovarian steroidogenesis,using isolated granulosa cells .Resultsshowed that IFN-a stimulated progesterone production in rabbit granulosa cells ina pose-and time-dependent manner and that the stimulatory effect of IFN-a wasblocked by condition of EGTA and verapamil.The results suggest that IFN-a playsa role in the regulation of rabbit ovarian steroidogenesis,via calcium dependent m-echanism.

关 键 词：干扰素 兔 颗粒细胞 孕酮 

分 类 号：R339.22[医药卫生—人体生理学]