人参二醇单体对大鼠心肌作用的单钙通道分析及ESR谱研究  

Single Calcium Channel Analysis and ESR Spectral Study on the Myocardial Effects of Panaxadiol Saponin Monomer in Rats

作　　者：孙成文[1] 赵春燕[1] 钟国赣[1] 王晓明[1] 江岩[1] 杨世杰[1] 

机构地区：[1]白求恩医科大学生理教研室,白求恩医科大学生理中心实验室,白求恩医科大学药理教研室

出　　处：《基础医学与临床》1994年第4期46-50,共5页Basic and Clinical Medicine

摘　　要：应用细胞封接式膜片钳方法，在Ｗｉｓｔａｒ大鼠的单个心室肌细胞上，观察了人参二醇组皂甙Ｒｃ（ｇｉｎｓｅｎｏｓｉｄｅ－Ｒｃ），Ｒｄ（ｇｉｎｓｅｎｏｓｉｄｅ－Ｒｄ）２００μｇ／ｍｌ对钙通道单通道活动的影响，并与钙通道阻滞剂异搏定３７．５μｇ／ｍｌ、钙通道激动剂ＢＡＹＫ８６４４μｍｍｏｌ／Ｌ对照，证明Ｒｃ对钙通道有阻滞作用；而Ｒｄ则无此作用。用电子自旋共振法（ｅｌｅｃｔｒｏｎｓｐｉｎｒｅｓｏｎａｎｃｅ，ＥＳＲ）测定了培养心肌细胞的自由基含量，Ｒｃ３０μｇ／ｍｌ能显著抑制黄嘌呤０．４２ｍｍｏｌ／Ｌ－黄嘌呤氧化酶５．３ｎｍｏｌ／Ｌ所致的自由基含量的增多：而Ｒｄ３０μｇ／ｍｌ对其影响则不明显。he effects of Rc, Rd 200μg/ml to single Ca2+channel on ventricular myocar-diocytes of Wistar rats Were recorded using the cell-attached configuration of thepatch clamp technique,and compared with calcium channel blocker verapamil 37.5μg/ml and calcium channel activator BAY K 8644 5μmmol/ml. Panaxadiol saponinmonomer Rc 200μg/ml inhibited the activities of T,L and B type of calciumchannel significantly,whill Rd 200μg/ml have no this effect. Electron spin reso-nance was used to measure the free radical contents of the cultured cardiomyocytes.Rc 30 μg/ml signif icantly reduce the increased free radical content indued by xanth-ine 0.42mmol/L-xanthine oxidase 5. 3nmol/L, but Rd 30μbg/ml no this effect.

关 键 词：钙通道 人参 皂甙 电子自旋共振 

分 类 号：R282.710.5[医药卫生—中药学]