吗啡对小鼠巨噬细胞吞噬、细胞毒作用及诱生细胞因子的影响  

Effects of Morphine on Phagocytosis, Cytotoxicity and Cytokines Production of Macrophages in Mice

作　　者：陆正武[1] 林志彬[1] 

机构地区：[1]北京医科大学基础医学院药理学系

出　　处：《基础医学与临床》1994年第5期31-34,共4页Basic and Clinical Medicine

摘　　要：小鼠递增剂量ｓｃ给予吗啡共４天，可建立稳定的躯体依赖模型。急性吗啡依赖使小鼠腹腔巨噬细胞（Ｍφ）吞噬中性红能力降低；使其对Ｓ１８０肿瘤细胞的细胞毒作用减弱；并平行性地抑制脂多糖（ＬＰＳ）诱导的白细胞介素１（ＩＬ－１）和肿瘤坏死因子（ＴＮＦ）的产生及其活性。纳洛酮可拮抗吗啡所致Ｍφ的功能抑制，而其单独给药对Ｍφ功能无明显影响。结果提示Ｍφ在阿片滥用所致免疫抑制的重要作用，且其体内效应可能通过阿片受体而介导。orphine was administered to mice sc in increasing doses for 4 days to causedependence, which was demonstrated by the significant loss of body weight andmost of the behavioral symptoms of abstinence syndrome after naloxone precipita-tion.The in vivo action of morphine and naloxone was investigated on peritonealmacrophages and supernatants collected from LPS-activated macrophages.It wasfound that macrophagde-mediated phagocytosing neutral red activity,cytotoxicitygaainst S180 cell,and IL-1/TNF levels in the supernatants of activated macropha-ges all decreased in morphine-treated mice. Conversely, groups of animals receiv-ed,either naloxone alone or morphine+naloxone,produced no suppression on ma-crophage function,though the dose of naloxone used to antagonize the action ofnorphine was only one tenth that of morphine, indicating that the in vivo effectof morphine on macrophage function was mediated by opioid receptor.Overall,hese observations suggest that morphine given in quantities sufficient to producedependence impairs in vitro parameters of immunocompetency in macrophages ,andthat the inhibition of macrophage functions may be an important cofactor in thepathogenesis of infectious diseases and tumors observed in opiate addicts.

关 键 词：吗啡 巨噬细胞 吞噬 细胞毒 细胞因子 

分 类 号：R996[医药卫生—毒理学]