冬眠和非冬眠期喜马拉雅旱獭乳酸脱氢酶同工酶的初步研究  

STUDY ON LDH ISOENZYMES OF THE HIMALAYAN MARMOT DURING HIBERNATION AND NON-HIBERNATION

作　　者：温得启[1] 刘国富[1] 黄孝龙[2] 

机构地区：[1]中国科学院西北高原生物研究所 [2]青海省地方病防治研究所

出　　处：《兽类学报》1989年第2期119-122,160,共4页Acta Theriologica Sinica

摘　　要：本文用聚丙烯酰胺凝胶盘电泳和紫外光谱法对冬眠期和非冬眠期喜马拉雅旱獭(Marmota himalayana)心、肾、肝脏及骨骼肌的乳酸脱氢酶同工酶带谱分布及其酶活力进行了测定。结果表明:(1)不同组织的酶谱有明显不同,且有多亚带和多态现象。(2)冬眠期各组织的总酶活力高于非冬眠期。The distribution of the lactate denydrogynase(LDH) isoenzyme bands in four tissues of the hibernation and non-hibernation himalayan maimot (Marmota himalayana) W2re analysed by means of the polyacrylamide gel disc electrophoresis and the o al LDH activity was detetmined. The resul s ob.ained aie as follows: 1. Five bands were found in kidney and skeletal muscle, from anode to cathode in turn were LDH-1. LDH-2, LDH-3, LDH-4 and LDH-5. Four bands were found in heart,and the proper order were LDH-1,LDH-2,LDH-3 and LDH-4.Only liver tissues of hibernation differ from the ones of non-hibernation, two bands(LDH-4 and LDH-5) were discovered only in liver tissues of hibeination himalayan marmot. However, four bands,LDH-2,LDH-3,LDH-4 and LDH-5 ware found in liver of non-hibernation.Sub-bands appeared in the LDH isoenzymcs of all other tissues except liver of hibeina-tion himalayan maimot. 2. Total enzyme activities of four tissues of hibeination ones were more or less higher than the ones of non-hibernation,but there was no significant difference (P> 0.05),Only in skeletal muscle there was significance(P<0.05)in terms of statistics.

关 键 词：喜马拉雅旱獭 LDH 同功酶 

分 类 号：Q959.839[生物学—动物学]