土壤微生物生物量氮测定过程中固铵问题  

AMMONIUM FIXATION DURING FUMIGATION ANDINCUBATION AFTER FUMIGATION IN SOILS

作　　者：殷士学[1] 王柏英[1] 宋琦[1] 胡健[1] 

机构地区：[1]江苏农学院农学系,江苏省镇江市农业局土肥站

出　　处：《江苏农学院学报》1994年第4期34-38,共5页Jiangsu Agricultural Research

基　　金：江苏省教委自然科学基金

摘　　要：土壤固铵作用对微生物生物量氮测定的影响结果表明，马肝土在熏蒸２４ｈ后的好气培养过程中交换性铵和固定态铵含量同步持续增多。到培养１０ｄ时固定态铵（氮）净增最为１７．３ｍｇ／ｋｇ，相当于此时交换性铵的４１．７％。在连续熏蒸１０ｄ过程中，第１ｄ熏蒸导致马肝土交换性铵和固定态铵明显升高，继续熏蒸基本不变，又一次显示交换性铵与固定态铵同步增加。熏蒸可导致硝态氮的损失，但损失主要发生在熏蒸的第１ｄ。用熏蒸－培养法和熏蒸－提取法测定５种土壤矿质氮激发量，结果表明土壤固铵能力都不同程度地影响矿质氮激发量的测定，影响程度因具体土壤的生物量和固铵能力而异。基于上述事实，作者建议现行的土壤微生物生物量氮计算方法作适当修改。The objective of this study is to determine the effect of ammonium fixation on the measurementof N flush by standard fumigation-incubation(FI)and fumigation-extraction(FE)methods.Resultsshowed that the fixed ammonium increased with the increase of extractable NH￣+ during either fumigation orincubation after fumigation.This phenomenon led us to conclude that the increase of fixed ammonium wasfrom the microbial cells.It is therefore suggested that the net ilncrease of fixed ammonium be included in theestimation of soil microbial biomass nitrogen by either Fl or FE method.

关 键 词：熏蒸 培养 提取 铵 固铵 生物量 氮 土壤 测定 

分 类 号：S158.2[农业科学—土壤学]