C_3植物叶片谷胱甘肽含量对光合逆境条件的反应  

Effects of Photoinhibitory Conditions on the Metabolism of Glutathione in C_3 Plant Leave

作　　者：熊福生[1] 宋平[1] 高煜珠[1] 

机构地区：[1]江苏农学院作物光合作用研究室

出　　处：《江苏农业学报》1994年第1期7-12,共6页Jiangsu Journal of Agricultural Sciences

摘　　要：Ｃ３植物大豆和棉花叶片中谷胱甘肽含量在自然条件（大田）下，每日早、晚相对稳定，午间呈明显增加趋势，日变化范围约０．５—０．８μｍｏｌ·ｇ－１ＦＷ；在光合逆境条件下，则迅速上升。增加光强，提高Ｏ２浓度，延长叶片暗（饥饿）处理时间以及外源Ｈ２Ｏ２、氨基三唑处理均明显导致叶片谷胱甘肽积累，表明谷－抗系统与植物体内光氧化产物的清除有关。同时也说明谷胱甘肽氧化还原反应受限于谷胱甘肽含量．并可能是光合逆境条件下谷－抗系统运行的限制步骤。By Ellman’s reagent-enzyme couple method,effects of photoinhibitory conditions on the metabolism of glutathlone in the leaves of C3 plants rice(Oryza sativa L.), soybean (Glycine max L.), peanut(Archis hypogaea L.) and cotton (Gossypium hirsutum L.)were investigated. The results showed that in the field on a typical sunny day, the total glutathione content in leaves of soybean and cotton in early morning and at dusk varied slightly, but in creased dramatlcally during noon(10: 00 16: 00). The diurnal change was about within 0.5 0.8 μmol· g-1FW.It was slso pbserved thst in CO2-free sir with stfong illumination,total glutathione in C3plants accumulated substantially,which can be simulated by raising O2 level and light intensity, elongating the time fof Plant starvation(dsrkening) and tfeating the plants with exogenous H2O2 and aminotriazole. Determination of GSH/GSSG+ GSH showed that the accumulation of total glutathione,most of which were GSH,resulted mainly from promotion of glutathione biosynthesis in leaves.The results above indicate that the glutathione content may be a real-limiting factor in the accelerated glutathioneascorbate cycle,which plays probably an important role in protecting C3 plants from photoin hibition.

关 键 词：C3植物 谷胱甘肽 光抑制 光合逆境 

分 类 号：S311[农业科学—作物栽培与耕作技术]