人IFNγ／TNFβ杂交蛋白的初步纯化及其抗癌效应研究  

Studies on the Preliminary Purification of Human IFNγ/TNFβHybrid Protein and Its Anti-Tumor Effect

作　　者：吴翰桂 丁仁瑞 马志章[1] 胡成均 吴雪昌[1] 徐庆乐 王首锋[1] 

机构地区：[1]浙江台州师专,杭州大学生物科学与技术系,浙江省卫生学校

出　　处：《科技通报》1994年第2期69-74,共6页Bulletin of Science and Technology

基　　金：国家自然科学基金

摘　　要：采用国产的微孔玻璃珠吸附层析法分离纯化人干扰素γ／肿瘤坏死因子β重组杂交蛋白，它具有抗病毒和抗肿瘤的双重功能活性，其抗病毒活性为１．７×１０￣６ｕ／ｍｇ蛋白，细胞毒活性为１．４×１０￣６ｕ／ｍｇ蛋白。该杂交蛋白在体外对人宫颈癌细胞株（ＭＥ１８０），人胃癌细胞株（７９０１）和人肺癌细胞株（Ｇ６）均有明显的杀伤作用，并都显著地高于单一的ＩＦＮγ或ＴＮＦβ对这些肿瘤细胞的杀伤。本文还对γＴＮＦβ的抑瘤机理进行了初步探讨。A recombinant hybrid protein-human interferonγ/tumor necrosis facter β(γTNFβ)was identified andpurified by means of micro-pore glass absorbed chromatography. It possesses dual functional activities of an-ti-virus and anti-tumor. The antivial and cytotoxic activities are 1.7 × 10￣6u/mg protein and 1.4 × 10￣6u/mgprotein respectively.The hybrid protein has appreciable killing effect against human tumor cell lines(such asME 180,7901 as well as G_6)in vitro and its cytotoxic action is significantly stronger than that of single IFNγor TNFβ.The anti-tumor mechanism of this hybrid protein had been investigated.

关 键 词：干扰素 重组蛋白 杂交 抗癌药 

分 类 号：Q513.03[生物学—生物化学]