苏木素硷性复红苦味酸法和肌红蛋白免疫组化法诊断心肌早期缺血的对比研究  

Comparison of the Haematoxylin Basic Fuchsin Picric Acid Method and the Immunohistochemical Stainning of Myoglobin for the Identification of Early Myocardial Ischemia

作　　者：张剑敏[1] 赵永和[1] 高津光洋 重田聪男 

机构地区：[1]昆明医学院法医学系,东京慈惠会医科大学法医学教室

出　　处：《昆明医学院学报》1994年第4期13-16,共4页Journal of Kunming Medical College

摘　　要：用改进的苏木素硷性复红苦昧酸法（ＨＢＦＰ）和肌红蛋白（Ｍｂ）免疫组化法对９例急性心肌梗塞（ＡＭＩ）和１１例其它原因猝死的８３块心肌组织的连续切片进行了对比研究．两种染色的阴性结果符合率达９５．２％，诊断心肌早期缺血改变的阳性率均为９１．３％，且ＨＢＦＰ阳性的心肌纤维分布在Ｍｂ脱失的范围内．作者认为，ＨＢＦＰ法除简便、快速、廉价以外，还有较好的灵敏度和可靠性，是诊断心肌早期缺血的良好辅助方法．eries of sections of 83 human heart tissue blocks from 9 cases of acute myocardial infarction(AMI)sudden death and 11 cases of non─AMI sudden death were examined with heamatoxylin ba-sic fuchsin picric acid(HBFP) method,ABC─immunohistochemical stainning of myoglobin (Mb),and HE,in order to clarify the reliability of HBFP for diagnosis of early myocadial ischemia.It wasdemonstrated that HBFP. and ABC─immunohistochemical stainning of Mb had the samepositivity of 9 l.3 % on sections of AMI cases, and among the sections with normalimmunohistochemical stainning of Mb, 95.2% sections were normal with HBFP futhermore,all of the HBFP positive myocardial flbres were observed in the area of Mb depletion.The re-sults of the present study indicate that HBFP is a reliable method for diagnosis of AMI.

关 键 词：心肌缺血 苏木素 硷性复红苦味酸 染色 肌红蛋白 

分 类 号：R541.404[医药卫生—心血管疾病]