聚合酶链反应检测心肌炎石蜡包埋组织柯萨奇病毒B_3 RNA  

Dection of Coxsackievirus B_3 RNA in Myocarditis from Paraffin-Embeded Heart Tissues by Polymerase Chain Reaction

作　　者：宫恩聪[1] 柯碧波 刘翠玲[1] 钟镐镐[1] 衡万杰[1] 刘淑平[1] 高子芬[1] 吴秉铨[1] 邱淑燕[2] 张礼和[2] 

机构地区：[1]北京医科大学病理学系,100083 [2]北京医科大学天然与仿生药物国家重点实验室,100083

出　　处：《临床与实验病理学杂志》1994年第4期286-289,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：报道从心肌炎石蜡包埋组织单一切片(6～8μm)中提取RNA,使用定位于柯萨奇病毒B_3特异编码区(VP_1)的一对引物、通过聚合酶链反应(PCR)进行基因扩增。对PCR扩增产物,采用3′末端生物素标记的柯萨奇病毒B_3的特异寡核苷酸探针进行斑点杂交。结果表明,两例检查的心肌炎标本都是柯萨奇病毒B_3感染引起的病毒性心肌炎。本实验说明,从石蜡包埋的组织标本中提取RNA,进行PCR检测是可行的,为临床病理进行分子生物学研究开辟了一条新途径。In this report, we showed that RNA isolated from myocarditis from paraf- fin-embedded single 6-8 μm heart tissue sections could be amplified by polymerase chain reaction (PCR) using a pair of primer located in a coxsackievirus B3-specific region (VP, gene). PCR products were detected by the specific oligonucleotide probes labeled with 3' terminal blotin for the coxsackievirus B3 genome on dot blots. Results demonstrated that two myocarditis tissue specimens were infected by coxsackievirus B3. This experiment suggests that RNA extracted from paraffin-embedded human tissues is amenable to analysis by PCR amplification. It provided a new means for molecular biology study of clinical pathology.

关 键 词：聚合酶链反应 病毒性 心肌炎 柯萨奇病毒B3 

分 类 号：R542.210.4[医药卫生—心血管疾病]