斑点免疫结合试验和酶联免疫吸附试验诊断包虫病的评价  

Evaluation of DIBA & ELISA in their Diagnosis of Human Hydatidosis

作　　者：王兰珍[1] 黄菱[1] 崔凯[1] 周悌[1] 张冬青[1] 

机构地区：[1]宁夏医学院寄生虫学教研室,宁夏医学院附属医院外科,宁夏医学院附属医院检验科

出　　处：《宁夏医学杂志》1994年第1期1-3,共3页Ningxia Medical Journal

摘　　要：用人肝棘球蚴液抗原对包虫病、非包虫病患者和健康人进行了斑点免疫结合试验（ＤＩＢＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）。结果显示，这两种酶免疫检测方法的敏感性分别为９８％和１００％，特异性分别为９８．７％和９８．１％，但ＤＩＢＡ具有节省抗原、试剂，不需特殊仪器设备，结果可长期保存等优点，更适用于临床诊断和流行病学调查。Dot-immunobinding assay(DIBA)and enzyme-linked immuno-sorbent assay(ELISA) were perfor-med with antigen from human hepatic hydatid fluid on sera of 51 patients with hydatid disease,81 patients of non-hydatid diseases and 73 healthy adults.Results showed that the sensi-tivity for the DIBA and ELISA were 98%and 100%respectively and the specificity were 98.7%and 98.1%res-pectively,t=1.02 and 0.42, P>0.05.The advantages of DIBA are more eco-nomical,simple,neednot any spe-cific instruments & equipment,andthe results could be kept for long time,thus,DIBA seems to be more suitable for clinical diagiiosis & epi-demiological survey.

关 键 词：棘球蚴病 诊断 人类 斑点 ELISA 

分 类 号：R532.320.4[医药卫生—内科学]