一种土壤真菌传小麦病毒的分离提纯及血清学研究  

Identification Purification and Serology of a Fungus-borne Wheat Virus

作　　者：邢怡明[1] 于嘉林[1] 晏立英[1] 李大伟[1] 蔡祝南[1] 刘仪[1] 

机构地区：[1]北京农业大学农业生物技术国家重点实验室

出　　处：《农业生物技术学报》1994年第1期91-95,共5页Journal of Agricultural Biotechnology

摘　　要：采田间患病小麦叶片，经ＰＥＧ沉淀、２０％蔗糖垫（含０．３％ＴｒｉｔｏｎＸ－１００）离心和氯化铯平衡密度梯度离心获得提纯病毒。紫外扫描呈典型核蛋白吸收曲线，Ａ２６０：Ａ２８０为１．１５。病毒粒子呈线条状，长度为２００～２０００ｎｍ。病毒核酸为两个组分。免疫电镜观察，小麦梭条斑花叶病毒（ＷＳＳＭＶ）抗血清和小麦黄花叶病毒（ＷＹＭＶ）抗血清均能强烈的“修饰”该病毒。提纯病毒免疫家兔，所得病毒抗血清效价可达１／１０２４，ＥＬＩＳＡ检测病毒灵敏度达０．１２８μｇ·ｍＬ－１。The virus was purified from infected wheat leaves by precipitating with PEG followed by differential contrifugation with 20%sucrose cushion comtaining 0.3%Triton X-100 and centrifuged in CsClisopycnic gradient.The uItraviolet absorption spectra of the purified virus preparation was typical of a filamen-tous virus and the A260:A280 ratio was 1.15.The particle length distribution of purfied virus was from 200 to2000 nm. It has two species of single-stranded RNAs and single coat protein with MWs of 28 kD. In ISEMtests,the virus particals were not only trapped effectively from saps but also decorated strongly by both WSS-MV-antisera and WYMV-antisera. Antiserum having a titer 1/1024 in micropreceptin test was obtainedfrom rabbits which had been immunized with the purified virus.The detection limition of the virus bv ELISAwas 0.128 μg/mL.

关 键 词：小麦 条斑花叶病毒 提纯 血清学 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]