一种高灵敏度的小檗碱高效液相色谱分析方法  

Analysis of Berberine by a Sensitive High Performance Liquid Chromatographic(HPLC)Method

作　　者：余琛[1] 洪有采 张慧[1] 徐修容[1] 

机构地区：[1]上海徐汇区中心医院,中国科学院上海药物所

出　　处：《色谱》1994年第1期37-39,共3页Chinese Journal of Chromatography

摘　　要：根据在卤代烃类溶剂中的小檗碱在紫外线的激发下可产生强烈的荧光这一特性，采用正相色谱－荧光检测的方法建立了小檗碱的高灵敏度检测方法。色谱柱为ＬｉｃｈｒｏｓｏｒｂＳＩ６０（５μ）２０ｃｍ×４．０ｍｍｉ．ｄ．。流动相为二氯甲烷－甲醇－二乙胺－冰醋酸（９０：９：０．４：０．５）。荧光检测波长Ｅｘ＝３６５ｎｍ，Ｅｍ＝５１０ｎｍ。方法的检测限为０．２ｎｇ（Ｓ／Ｎ≥２．５），在０．２ｎｇ～２０ｎｇ范围内具有良好的线性关系。n halogenated solvents, strong fluorescence emits from berberine under UV radiation, According to thischaracteristic a sensitive assay method was developed by using normal phase HPLC and fluorescence detection.Silica gel(Lichrosorb SI 60(5μ)was used as the stationary phase and a mixture of dichloromethane-methanol-diethyl amine-acetic acid(90 :9 :0.4:0.5)as the mobile phase, The filter of fluorometer was 510nm(excition at365nm).The limit of detection was 0. 2ng(S/N≥2.5)and the range of linearity was 0.2～20ng with acorrelation coefficient of 0.9999.

关 键 词：小檗碱 高效液相色谱 荧光 黄连 

分 类 号：Q946.88[生物学—植物学]