离子对高效液相色谱－磺化四苯基卟啉光度法同时测定痕量锌和铜的研究  

Simmtaneous Spectrophotometrlc Determination of TraceAmounts of Zn(Ⅱ)and Cu(Ⅱ)with Meso-Tetra-(4-Sulfophenyl)Porphyrin by Ion-pair High Performance Llquid Chromatography

作　　者：雷呈宏 汤福隆[1] 汤淼荣 陈秀华[1] 

机构地区：[1]杭州大学化学系

出　　处：《色谱》1994年第4期276-278,共3页Chinese Journal of Chromatography

基　　金：省自然科学基金资助课题

摘　　要：用ＴＰＰＳ＿４作柱前衍生试剂，寻找到试剂与锌（Ⅱ）和铜（Ⅱ）的最佳反应条件，然后在ＯＤＳ柱上，以乙腈－水体系（２４：７６，ｖ／ｖ）作流动相，四乙基碘化按作离子对试剂，在４１５ｎｍ处检测，确立了离子对高效液相色谱－光度法快速分离测定痕量锌和铜的新方法，检测下限（×ｌ０￣（－９）ｇ／ｍＬ）：Ｚｎ０．１０，Ｃｕ０．０９。所建立的方法用于花生样品中锌和铜的测定，结果令人满意。nly by heating at pH 4.4 chelating reaction of meso-tetra(4- sulfophenyl)porphyrin(TPPS_4)with Zn(Ⅱ)and Cu(Ⅱ) can be completed. ZnTPPS_4, TPPS_4 and CuTPPS_4 were separated within 8 min on an ODScolumn and eluted with acetonitrile+water(24 : 76,V/V)containing 50 mmol/L tetraethylammonium iodideand l0 mmol/L acetic acidsodium acetate buffer solution(pH 6, 4), and detected at 4l5 nm。 The detectionlimits were 0.l and 0. 09(×10￣(-9)g/mL)for Zn and Cu respectively. This method can be used for the deter-mination of trace Zn and Cu in the peanut sample.

关 键 词：高效液相色谱 锌 铜 花生 TPPS4 

分 类 号：S565.2[农业科学—作物学]