高效液相色谱和高效液相色谱－质谱法测定粮食中互隔交链孢霉醇、互隔交链孢霉醇单甲醚及玉米赤霉烯酮  

Analysis of Alternariol,Alternariol Monomethyl Ether and Zearalenone in Cereals by High Performance Liquid Chromatography(HPLC)and Particle Beam Liquid Chromatography/Mass Spectrometry(LC/MS)

作　　者：罗毅[1] 刘锋[1] 冯建林[1] 张理汉[1] 胡绪英[1] 

机构地区：[1]北京军事医学科学院毒物药物研究所

出　　处：《色谱》1994年第5期342-344,347,共4页Chinese Journal of Chromatography

摘　　要：建立了粮食中互隔交链孢霉醇（ＡＯＨ）、互隔交链孢霉醇单甲醚（ＡＭＥ）和玉米赤霉烯酮（ＺＥＡ）三个毒素的ＨＰＬＣ定性定量分析法和粒子束ＬＣ－ＭＳ／ＥＩ￣＋鉴定方法。采用反相色谱，以８０％的甲醇水溶液作流动相，这三个毒素在Ｃ＿（１８）色谱柱上彼此间均获得较好的分离。方法对粮食中ＡＯＨ，ＡＭＥ两个毒素的回收率均在７９％以上。ＡＯＨ，ＡＭＥ两个毒素的最低检出限为１×１０￣（－９）ｇ。用所建方法对１００多个大骨节病病区、非病区的粮食样品进行了检测，结果为阴性。对７个互隔交链孢霉菌培养物检测的结果表明这些培养物中不同程度地产生了ＡＯＨ，ＡＭＥ两个毒素，含量为１３．０～９５．９ｍｇ／ｋｇ。并用粒子束ＬＣ－ＭＳ技术成功地对这两个毒素进行了鉴定。qualitative and quantitative method for simultaneously analyzing alternariol(AOH),alternariolmonomethyl ether(AME)and zearalenone(ZMA)in cereals and cultures by HPLC and particle beam LC/MSwas established, These three toxins were effectively separated on a C_(18) reversed-phase column with an 80%aqueous methanol as the eluting agent.The recoveries of the three toxins in corn and wheat were all over79%. The detection limits for them were 1×10￣(-9)g. With this method, about 100 cereal samples collectedfrom the disease and non-disease areas of Kashin-Beck disease were detected.None of the three toxins wasfound. However,AOH and AME were detected in seven cultures of the genus Alternaria by HPLC and weresuccessfully identified by particle beam LC/MS.The contents of AOH and AME in these cultures were in therange from 13.0mg/kg to 95.5mg/kg.

关 键 词：粮食 AOH AME ZEA 高效液相色谱 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]