高效液相色谱法测定大鼠肺及脑组织中细胞膜磷脂  

Determination of Phospholipid in Membrane of Rat Lung andBrain Tissues by High Performance Liquid Cbromatography(HPLC)

作　　者：周建忠[1] 邢成[1] 王鲁明[1] 

机构地区：[1]军事医学科学院仪器测试中心

出　　处：《色谱》1994年第6期441-442,共2页Chinese Journal of Chromatography

摘　　要：高效液相色谱法测定大鼠肺及脑组织中细胞膜磷脂周建忠，邢成，王鲁明（军事医学科学院仪器测试中心北京１００８５０）１前言细胞与周围环境的联系和反应都必须通过细胞膜来完成￣［１］．磷脂是细胞膜的重要组成，它对细胞的功能起着重要作用￣［２］。目前测定磷脂的方...A successful method for separation and quantitative determination of phosphatidylinositol(PI),phos-phatidylserine(PS),phosphatidylethanolanune(PE)and phosphatidylcholine(PC) in membrane of rat lungand brain tissues by HPLC has been developed.Under the selected conditions ,the averagerecoveries of themwere 84.14～88.43%, The contents of PI, PS, PE and PC(mean±SD)per gram of tissues in the mem-brane of normal rat lung were 201.1±20.8μg,350.6±20.0μg,162.0±11. 6μg and 24.66±2.04mg respec-tively. In the membrane of normal rat brain,the contents of them were 425.2 ± 30.2μg,2862 ± 326μg,878.4±55.7μg and 32. 83±3.73mg per gram of tissues respectively.

关 键 词：高效液相色谱 大鼠 肺 脑组织 细胞膜磷酯 

分 类 号：Q545[生物学—生物化学]