t－pA　ELISA测定方法的建立和应用  

Establishment and Clinical Application of An Enzyme-liked Immunosorbent Assay (ELISA)for Determination of Tissue-type Plasminogen Activator (t-PA)Antigen in Humn Plasma

作　　者：张启良[1] 陈岚[1] 杨君蕊[1] 倪玉英[1] 

机构地区：[1]上海第二医科大学病理生理教研室

出　　处：《上海第二医科大学学报》1994年第4期308-312,共5页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：本文建立了测定血浆组织型纤溶酶原活化素（ｔ－ＰＡ）抗原的ＥＬＩＳＡ法，分析以该法测定ｔ－ＰＡ的最小可测浓度、回收率、稳定性和特异性，还与市售同类试剂盒同时测定的３５份血浆ｔ－ＰＡ浓度作比较。结果：测定１１１名中青年正常人的血浆ｔ－ＰＡ，均值为４．０８±２．５９μｇ／Ｌ，１３名高龄正常人平均为１７．６３±８．１５μｇ／Ｌ，明显增高（Ｐ＜０．００１）。正常人一日内血浆ｔ－ＰＡ以上午８时较高，下午４时较低；静脉阻断试验后ｔ－ＰＡ水平可明显增高（Ｐ＜０．００１）。另外，测定了９３名包括肾疾患、高血压、冠心病、急性心肌梗塞和脑梗塞以及深静脉血栓形成患者的血浆ｔ－ＰＡ，对测定结果作了分析和讨论。An enzyme-linked immunosorbent assay (ELISA) for t-PA antigen determination in human plasma has been devised according to the Sandwich method using rabbit antibody to human t-PA antigen. The minimal measurable concentration was 0.0625μg/L,the mean recovery was 100.34% and coefficients of varation for the within and between-assay were 3.83% and 7.81% respectively. No reaction could be detected when the u-PA,PF4 or βTG was analyzed by this ELISA method. Results showed that the mean plasmat-pA level in 13 elderly healthy volunteers was significantly higher than that in 111 healthy youngadults(P<0.01)In24 normal adults the plasma t-PA levels were higher at 8 am vs 4 pm,and the levelelevated significantly 10 min after venous occlusion compared with that prior to the test(P<0.01).Besides,the t-PA levels in 93 cases patients suffering from various diseases,including renal diseases, coronary heart disease, acute myocardial infarction, acute cerebral infarction,deep vein thrombosis,were also determined and descussed.

关 键 词：纤溶酶原活化素 急性 心肌梗塞 ELISA测定 

分 类 号：R446.61[医药卫生—诊断学] R541.03[医药卫生—临床医学]