共染ANP对GH_4和AtT_（20）细胞肾素基因表达分泌的影响  

Inhibition of Renin and Prorenin Release in GH4 and AtT20 Cells Cotransfected by ANP and Renin Genes

作　　者：苏永新[1] 付爱芬[1] 张梅[1] 王莉[1] 

机构地区：[1]上海第二医科大学病理学教研室

出　　处：《上海第二医科大学学报》1994年第4期304-307,共4页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：本文应用现代基因共同转染技术，首次对ＧＨ４和ＡｔＴ２０细胞进行了重组大鼠ＡＮＰ和人肾素基因的共同转染，建立了表达细胞模型。并对其表达产物进行分析研究。文章着重分析研究了ＡＮＰ对肾素基因的表达产物的形成与分泌的调节作用。ＡＩ形成放免检测结果表明：转染细胞的表达是有效的。转染的ＧＨ４能有效地表达分泌肾素原；转染的ＡｔＴ２０细胞则不仅能形成肾素原，而且能分泌具有活性的肾素。ＡＮＰ转染的实验组不仅显示有明显的拮抗Ｆｏｒｓｋｏｌｉｎ的刺激作用，而且ＡＮＰ对肾素与肾素原的形成分泌有抑制作用，其中抑制肾素分泌的效能大于肾素原的分泌。实验证明，转染基因的表达细胞是有效研究肾素基因表达、分泌等调节的细胞模型。By means of gene cotransfection technique, GH4 and AtT20 cells were cotransfected by recombinant rat atrial natriuretic peptide(ANP)gene and human renin gene, and the expressed products were studied.The radioimmunoassay of AI formation showed that the expression of transfected cells were effective.The only renin gene transfected GH4 cell could express prorenin efficiently,while the only renin gene transfected AtT20 cellcould not only produce pro-renin but also secret bioactive renin, However, both ANP and renin genes cotransfectd GH4 and AtT20 cell showed a low secretion of prorenin and renin indicating that the expressed ANP inhibited the renin and prorenin release, the inhibitory rate of renin rlease was more than that of prorenin. The cotransfected cells also showed that the forskolin stimulation of renin release was suppressed. It is therefore apparent that cotransfected cells are satisfactory models for studies of renin gene expression,secretion and regulation.

关 键 词：肾素 ANP 基因转染细胞 表达分泌调节 病理生理 

分 类 号：R392.32[医药卫生—免疫学]