不同激素配比对石刁柏愈伤组织诱导生长和多糖含量的影响  

EFFECTS OF DIFFERENT GROWTH REGULATORS ON INDUCTION,GROWTH AND POLYSACCHARIDE AMOUNT OF ASPARAGUS CALLI

作　　者：蒋琳[1] 陆仕华[1] 周正来[1] 沈革志[1] 韩佩来[1] 

机构地区：[1]上海市农业科学院植物保护研究所,上海市农业科学院农业生物技术研究中心

出　　处：《上海农业学报》1994年第4期89-92,共4页Acta Agriculturae Shanghai

摘　　要：以（ＭＳ＋各种激素）作石刁柏愈伤组织诱导和继代的培养基，比较了不同器官外植体对诱导愈伤组织的影响。结果表明，石刁柏幼茎段在ＭＳ＋２，４－Ｄ（０．５）＋ＢＡ（１．０）培养基上，愈伤组织诱导率达１００％，且质地较疏松；ＭＳ＋２，４－Ｄ（１．０）＋ＢＡ（０．２）作继代培养基，愈伤组织增殖率最高，并在主愈伤组织块周围形成疏松浅黄色愈伤组织颗粒；在各部位外植体（茎顶芽、下胚轴、根、茎中段）诱导的愈伤组织中，以茎中段诱导的愈伤组织频率最高，多糖含量也最高。In this study, Asparagus officinalis L. calli were induced from different explants and then subcultured using different growth regulators. The results showed that the rate of induced calli growing on the medium of MS+2, 4-D(0. 5)+BA (1. 0) was 100%, but the texture of calli was friable. The calli grew well on the subculture medium of MS+2, 4-D (1. 0) +BA (0. 2) and light yellow grains were formed on the surface of calli. These calli grew most rapidly. Among the calli induced from different explants, the calli from shoots showed the highest rate of induced calli and had the highest amount of pogysaccharidws.

关 键 词：石刁柏 愈伤组织 生长调节剂 多糖 激素 

分 类 号：S644.603.5[农业科学—蔬菜学]