抗人IgG单克隆抗体制备和鉴定  

PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO HUMAN IgG

作　　者：丁皓[1] 吴晓俐[1] 朱运松[1] 宋后燕[1] 

机构地区：[1]上海医科大学基础医学院分子遗传学研究室

出　　处：《上海医科大学学报》1994年第3期165-169,共5页Journal of Fudan University(Medical Science)

摘　　要：以人ＩｇＧ为抗原，采用杂交瘤技术获得６株不同性质的抗人ＩｇＧ单克隆抗体（ＭｃＡｂ）（ＡＩ２、ＡＩ３、ＡＩ４、ＡＩ５、ＡＩ６、和ＡＩ）。ＡＩ２ＭｃＡｂ具有抗ＩｇＧ１亚类的特异性，可识别ＩｇＧγ链和Ｆ（ａｂ＇）２片段，作用位点可能在人ＩｇＧ１铰链区。ＡＩ３ＡＩ、ＭｃＡｂ分别为ＩｇＧ３、ＩｇＧ４亚类限制性ＭｃＡｂ，可能作用于ＩｇＧＣＨ１区。ＡＩ４ＭｃＡｂ可识别χ和λ型ＩｇＧ四个亚类以及Ｆ（ａｂ＇）２片段，不识别游离χ、λ链。ＡＩ５ＭｃＡｂ特异地抗ＩｇＧ１结合型χ，作用于ＩｇＧ独特型位点。ＡＩ６ＭｃＡｂ只抗λ型ＩｇＧ，可能作用于ＩｇＧＣＬ区。６种抗人ＩｇＧＭｃＡｂ除了ＡＩ５外，均能用于人血清中ＩｇＧ的检测。Six monoclonal antibodies(AI2、AI3、AI4、AI5、AI6、and AI7) which against human IgG were obtained by fusion of mouse myeloma cells(SP2/0) and spleen cells previously immunized with human IgG. AI2 McAb had a specificity to IgG1 ang recognized γ chain of IgG and F(ab')2 fragment. Its antigenic determinant may be located in IgG1 hinge region.AI3、AI7 McAb were IgG3、IgG4 subclassrestricted specificity, respectively.They would directly against IgG CH1 region. AI4 McAb recognized four subclasses of human IgG(χ、λ) and F(ab')2 fragment,not free χ、λ chain. AI5 McAb specially recognized χ chain linked to IgG1.It was ideotype-specific.AI6 McAb only recognized IgG(λ),It would directly against IgG CL region.Besides AI5, all the McAbs could used to detect the specific IgG in human serum.

关 键 词：单克隆抗体 免疫球蛋白G 制备 

分 类 号：R392－33[医药卫生—免疫学]