胆汁中70kD促成核糖蛋白的分离、提纯及其理化性质的研究  

ISOLATION AND PURIFICATON OF A 70kD PRONUCLEATION GLYCOPROTEIN IN BILE AND ITS PHYSICOCHEMICAL CHARACTERISTICS

作　　者：焦宛[1] 张延龄[1] 吴树强[1] 蔡端[1] 

机构地区：[1]上海医科大学附属华山医院外科教研室

出　　处：《上海医学》1994年第4期187-190,共4页Shanghai Medical Journal

基　　金：国家自然科学基金

摘　　要：胆汁中促抗成核因子的发现是近年来胆石成因研究中的一大进展。我们应用ＣｏｎＡ固相化亲和层析结合ＳｅｐｈａｄｅｘＧ－２００分子筛色谱技术，首次从人体胆汁中分离、纯化到一种纯的糖蛋白，表现分子量为子量的此７０ｋＤ，具有明显的促成核活性，其中性己糖与蛋白质含量之比为１５．８８％，一分糖蛋白约含６４０只氨基酸，其Ｎ－末端氨基酸顺序为Ｎ—Ｓｅｒ—Ｇｌｙ—Ｍｅｔ—Ａｒｇ—Ｇｌｎ—Ｔｙｒ—Ｍｅｔ—Ｌｙｓ。本文同时讨论了此糖蛋对胆石成因的病理学和生物学意义。n recent years,the big achievement in studying the etiology of cholelithiasis isthe recognition of the role played by the pro and anti-nucleating factors in human bile.Using ConA affinity chromotography combined with Sephadex-200 molecular sieve tech-nique,we have successfully isolated and purified a glycoprotein in gallbladder bile,Ithas obvious pronucleating capicity,and the quantity ratio of neutral hexose to proteinis 15.88%.One molecule of it contains about 640 amino acid residues,The N-terminalamino acid sequence is N-Ser-His Gly-Met-Arg-Cln-Tyr-Tyr-Met-Lys.The pathogenicand biological significance of this glycoprotein in the gallstone pathogenesis have beendiscussed.

关 键 词：促成核因子 糖蛋白 胆结石 

分 类 号：R657.42[医药卫生—外科学]