蛹草菌菌丝体培养条件的选择  

A Selection of External Factors for Growth of Mycelia of Cordyceps militaris (L.) Link

作　　者：华秀英[1] 曹杰[1] 

机构地区：[1]沈阳农业大学土化系

出　　处：《沈阳农业大学学报》1994年第3期303-307,共5页Journal of Shenyang Agricultural University

摘　　要：不同培养基、环境因素（温度、ｐＨ、光照）、营养条件（碳源、氮源）对蛹草菌菌丝体生长影响的研究结果表明：蛹草菌菌丝体在ＰＤＡ综合培养基和沈农５号培养基上生长状况最好，在ＰＤＡ培养基、ＰＤＡ麸皮培养基生长最差．菌丝体在１５～２５℃范围内均能生长，其中在２５℃条件下生长状况最好，而且随着温度的升高，生长状况明显增强，３０℃和件下生长成侵，３５℃停止生长．菌丝体对酸碱度要求不严，在ｐＨ５．０～８范围内均能生长，其中在ｐＨ６．５～７．０生长最好．菌丝体在非光照条件下比在光照条件下生长旺盛．对不同碳源、氮源吸收各异，其中以甘露醇为碳源，以牛肉膏为氮源生长状况最佳．The results showed that mycelia of Cordyceps militaris could grow well on PDA composite and No. 5 of Shennong culture mediums, whereas grew worse on the culture mediums of PDA and PDA with bran. The mycelia would grow at 15￣25℃, the bet at 25℃,but grew slowly at 30℃ and could not glow at 35℃. At PH 5.0￣8.0, there mycelia also would grow, but the best range of pH was at 6.5￣7.0. They grew better in dark condition than in light. The best carbon source was mannitol and the best nitrogen source was extract beef.

关 键 词：外界因子 固体培养基 蛹草菌 冬虫夏草 

分 类 号：S567.35[农业科学—中草药栽培]