组织培养生产高山红景天有效成分的研究　Ⅰ．高山红景天愈伤组织的诱导与培养  

Studies on Producing Effective Medicinal Ingredients of Rhodiola sachalinensis by Tissue CultureⅠ.Callus Induction and Culture of Rhodiola sachalinensis

作　　者：姜明兰[1] 钟文四 韩洪[1] 刘声远[1] 孟庆勇[1] 程国华[1] 

机构地区：[1]沈阳农业大学遗传研究所,沈阳农业大学生物化学教研室

出　　处：《沈阳农业大学学报》1994年第4期355-359,共5页Journal of Shenyang Agricultural University

基　　金：辽宁省科委重点课题

摘　　要：以高山红景天的叶片、子叶、茎和根为外植体。诱导产生了大量的愈伤组织．筛选出适于愈伤组织大量培养的固体培养基．以６ＢＡ４～８ｍｇ／ｌ，ＮＡＡ２～２．５ｍｇ／ｌ为最佳激素种类和浓度．研究了固体培养条件下愈伤组织生长规律，绘制了生长曲线，并筛选出优良的细胞无性系．The callus was induced and cell culture system was established with a piece of leaf,rotyiedon,stem and root of Rhodiola sachalinensis.The experiments showed that good callus growth was obtained on a medium containing both BA 4 ￣ 8mg/l and NAA 2～2.5mg/l.The pattern of callus glowth was found out and the good cell clones were selected.

关 键 词：高山红景天 组织培养 愈伤组织 

分 类 号：S336[农业科学—作物遗传育种] S567.239[农业科学—农艺学]