大鼠自发性高血压与HSP70基因关系的研究  

RELATION OF HSP70 GENE AND SPONTANEOUSLY HYPERTENSIOW IN RATS

作　　者：黄生宁[1] 杨鸿[1] 姚阿卿 张晨晖[1] 温进坤[1] 唐朝枢[1] 周爱儒[1] 汤健[1] 

机构地区：[1]北京医科大学心血管基础研究所

出　　处：《生理学报》1994年第3期288-292,共5页Acta Physiologica Sinica

摘　　要：本实验采用热休克蛋白７０（ＨＳＰ７０）核酸分子杂交方法，检测了自发性高血压大鼠（ＳＨＲ）和正常血压大鼠（ＷＫＹ）离体培养的主动脉平滑肌细胞（ＡＳＭＣ）受热刺激后ＨＳＰ７０ｍＲＮＡ水平的变化以及整体动物肝组织ＨＳＰ７０ｍＲＮＡ的水平，并对肝组织基因组ＤＮＡ进行了限制性酶切片段长度多态性（ＲＦＬＰ）分析。结果表明：３７℃培养的ＳＨＲＡＳＭＣ及整体ＳＨＲ肝组织ＨＳＰ７０ｍＲＮＡ的基础表达水平均低于ＷＫＹ鼠，ＳＨＲＡＳＭＣ受热刺激（４２℃１５ｍｉｎ）后２ｈ，ＨＳＰ７０ｍＲＮＡ表达增加的程度明显大于ＷＫＹ鼠。肝组织基因组ＤＮＡ经ＢａｍＨＩ酶切后，ＳＨＲＨＳＰ７０基因缺失一条约５．６ｋｂ的片段。本结果提示ＳＨＲＡＳＭＣ对热敏感，ＨＳＰ７０基因异常可能与高血压发生有关。The level of heat shock protein 70 messenger RNA was examined in aorticsmooth muscle cells(ASMC)and hepatic tissue of Wistar-Kyoto(WKY)rats andspontaneously hypertensive rats(SHR).The restriction fragment length polymorphism(RFLP)was also analysed in hepatic DNA.It was demonstrated that the basiclevel of heat shock protein 70messenger RNA in ASMC and heretic tissue of SHRwere lower than that of the WKY rats.The extent of heat shock Plotein 70 messengerRNA accumulated in ASMC was higher in SHR than in WKY rats when the cells wereheated by immersing the culture bottles in 42℃ water bath for 15 min and allowed torecover at 37℃ for 2 h. In contrast with WKY rats,the Bam HI restricted bepeticDNA showed that a fragment about 5. 6 kb was lost in heat shock protein 70 genefrom hepatic DNA of SHR.The results suggest that the ASMC of SHR is more sensitive to heat stress than that of WKY rats and the changes of heat shock protein gene sequence may be related to genetic hypertension.

关 键 词：高血压 热休克蛋白 RFLP 基因 

分 类 号：R544.102[医药卫生—心血管疾病]