一氧化氮合成酶（NOS）基因表达的半定量检测及其运用  

SEMIQUANTITATIVE DETECTION AND APPLICATION ABOUT THE EXPRESSION OF NITRIC OXIDE SYNTHASE GENE

作　　者：张晨晖[1] 李倩虹[1] 张继峰[1] 周洪[1] 张新波[1] 汤健[1] 

机构地区：[1]北京医科大学心血管基础研究所分子生物学研究室

出　　处：《生理学报》1994年第4期347-354,共8页Acta Physiologica Sinica

摘　　要：本工作参照Ｍａｒｔｉｎ（１９９３）定量ＲＴ－ＰＣＲ方法，建立了一种灵敏、简捷、特异的定量ＮＯＳｍＲＮＡ测定方法；证明了ＮＯＳｍＲＮＡ不仅存在于脑组织，亦广泛分布于心、肾、肺和肝组织中，其中以脑组织含量最高，肾、心次之。除内皮细胞以外，平滑肌细胞中亦有ＮＯＳ基因的表达；此外，本工作还观察到，自发性高血压大鼠的脑、肾、肝和平滑肌细胞中ＮＯＳｍＲＮＡ水平下降，提示ＮＯＳ基因表达受抑，可能与高血压的病因密切相关。Using the Reverse Transcription (RT)-Polymerase Chain Reaction (PCR) method of Martin (1993) for semiquantitation determinating of NOS gene, it was found that NOS mRNA is not only existed in brain, but also distributed extensively in heart, kidney, lung and liver. Among of them, NOS mRNA levels were highest in the brain, followed in descending order by kidney, heart. In addition to endothelial cell, NOS gene was also highly expressed in smooth muscle cells, suggesting that they may be an important site of NOS in organism. Furthermore, NOS mRNA levels were found to decrease significately in brain, kidney, liver and smooth muscle cell in spontaneous hypertensive rat. These data suggest that pathogeny of hypertention may be related to low expression of NOS gene in these tissues.

关 键 词：一氧化氮 合成酶 基因表达 高血压 

分 类 号：R544.102[医药卫生—心血管疾病]